Anti-DKK3 Rabbit Monoclonal Antibody for WB/IF/ICC/ELISA - Q9UBP4

Core Product Specifications and Parameters

Biological Background: DKK3 Function and Localization

• DKK3 (Dickkopf-related protein 3), also known as REIC or RIG, is a secreted glycoprotein belonging to the Dickkopf family of Wnt antagonists. It contains a signal peptide, two cysteine-rich domains, and coiled-coil regions.
• DKK3 antagonizes canonical Wnt/β-catenin signaling by binding to LRP5/6 co-receptors and promoting their internalization via KREMEN transmembrane proteins, thereby inhibiting Wnt ligand-receptor interaction.
• This protein plays critical roles in embryonic development, including anteroposterior axial patterning, limb morphogenesis, somitogenesis, and eye formation, by locally modulating Wnt activity.
• In adult tissues, DKK3 is implicated in bone homeostasis, tumor suppression (frequently downregulated in cancers), and neurodegenerative processes such as Alzheimer disease, though the latter functions are inferred from similarity.
• Subcellular localization is exclusively extracellular: DKK3 is secreted into the extracellular space and does not appear to be membrane-bound or intracellular.
• Expression is highest in heart, brain, and spinal cord, with lower levels in other tissues, suggesting tissue-specific regulatory functions.
• Post-translational modifications include N-glycosylation and disulfide bond formation, which are typical for secreted cysteine-knot proteins and may affect its stability and activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 200–350 of human DKK3, covering the C-terminal region rich in coiled-coil motifs. This region may detect full-length DKK3 and potentially some cleavage products; verify the molecular weight in your system.
• For Western blot (WB), the predicted molecular weight is approximately 38 kDa (calculated from amino acid sequence). Glycosylation may cause the observed band to migrate slightly higher. Use appropriate controls.
• For immunofluorescence/immunocytochemistry (IF/ICC), as DKK3 is a secreted protein, including a Golgi-blocking agent (e.g., BFA) may enhance intracellular staining if retention is desired; otherwise, consider analyzing secreted DKK3 in cell supernatants via ELISA.
• The antibody is tested for ELISA applications; it can be used for antigen capture or detection depending on the assay format. Perform cross-reactivity tests with your particular matrix.
• Despite reported cross-reactivity with mouse and rat, the sequence homology in the immunogen region is high; still, validate reactivity in species-specific tissues.
• Always titrate the antibody in your assay conditions for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one-stop partner for IVD raw-material sourcing, supporting diagnostic manufacturers and research labs from concept to clinic. For Wnt pathway targets like DKK3, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assistance in rare target procurement. Let us streamline your development pipeline with reliable, high-quality materials tailored to your needs.