Anti-DKK3 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UBP4

Core Product Specifications and Parameters

Biological Background: DKK3 Function and Localization

• Dickkopf-related protein 3 (DKK3) is a member of the dickkopf family of secreted Wnt antagonists, encoded by the DKK3 gene (also known as REIC).
• DKK3 antagonizes canonical Wnt signaling by inhibiting the interaction of LRP5/6 co-receptors with Wnt ligands, and by forming a ternary complex with KREMEN that promotes LRP5/6 internalization.
• DKK proteins are critical in vertebrate development, locally inhibiting Wnt-regulated processes such as antero-posterior axial patterning, limb development, somitogenesis, and eye formation.
• In adults, DKK3 is implicated in bone formation and bone disease, cancer, and Alzheimer’s disease (By similarity).
• DKK3 is a secreted glycoprotein with predicted coiled-coil domains and disulfide bonds.
• Tissue expression is highest in heart, brain, and spinal cord.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 22-200 of human DKK3, suggesting the antibody may recognize multiple epitopes along the N-terminal region.
• For Western blot, the predicted molecular weight is approximately 38 kDa; consider using appropriate reducing conditions.
• In immunofluorescence/immunocytochemistry, ensure proper fixation and permeabilization to detect the secreted protein.
• The antibody cross-reacts with mouse and rat DKK3, which can be validated in the relevant sample systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Whether you require anti-DKK3 antibodies for Wnt signaling research or cancer biomarker development, our one-stop sourcing bridge connects you to high-quality reagents and tailored technical consulting. We also specialize in sourcing rare target raw materials to accelerate your projects from concept to clinic.