Anti-DKK1 Rabbit Polyclonal Antibody for WB and ELISA - O94907

Core Product Specifications and Parameters

Biological Background: DKK1 Function and Localization

• DKK1 (Dickkopf-related protein 1), also known as SK, is a secreted glycoprotein and a key antagonist of the canonical Wnt signaling pathway.
• It functions by binding to LRP5/6 and Kremen proteins, forming a ternary complex that promotes LRP5/6 internalization and blocks Wnt signal transduction. Related references: PMID:22000856
• DKK1 plays critical roles in embryonic development, including antero-posterior axis patterning, limb development, somitogenesis, and eye formation.
• In adults, DKK1 is implicated in bone metabolism, cancer progression, and Alzheimer's disease. Related references: PMID:17143291
• The protein is predominantly secreted and exhibits placenta-specific expression.
• It contains conserved disulfide bonds and is subject to glycosylation, as identified by proteomics and structural studies.
• Additionally, DKK1 can inhibit the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, conferring anti-apoptotic activity (by similarity).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 32–266 of human DKK1, covering the mature secreted protein; consider this when planning epitope mapping or blocking peptide experiments.
• For Western blotting, use a reducing SDS-PAGE protocol and validate the band at ~29 kDa; include positive controls from human, mouse, or rat tissue lysates.
• In ELISA, a purified recombinant DKK1 protein standard is recommended for generating a calibration curve; validate cross-reactivity in your specific sample matrix.
• Confirm antibody specificity using siRNA knockdown or knockout cell lines if necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material supplier, offering antibodies, antigens, and ancillary reagents essential for assay development. For researchers investigating Wnt signaling and developmental biology, we provide validated anti-DKK1 antibodies, bulk raw materials, and technical support to streamline your diagnostic or research workflows. Contact us for customized sourcing solutions that bridge the gap from concept to clinic.