Anti-DKC1 Rabbit Polyclonal Antibody for WB - O60832

Core Product Specifications and Parameters

Biological Background: DKC1 Function and Localization

• DKC1 is the catalytic subunit of the H/ACA small nucleolar ribonucleoprotein complex, catalyzing pseudouridylation of rRNA, which stabilizes rRNA conformation and is essential for ribosome function. Related references: PMID:25219674 PMID:32554502
• It is required for ribosome biogenesis and telomere maintenance, particularly by ensuring correct processing and intranuclear trafficking of TERC, the RNA component of telomerase. Related references: PMID:19179534 PMID:25219674
• The enzyme catalyzes the isomerization of uridine to pseudouridine by attaching the ribose to C5 instead of N1, a modification that can occur at up to 100 residues per rRNA molecule. Related references: PMID:25219674
• DKC1 localizes to the nucleolus and Cajal bodies in the nucleus, and also appears in the cytoplasm, consistent with its roles in snoRNP assembly and trafficking.
• Ubiquitously expressed across human tissues, reflecting its fundamental housekeeping function.
• Additional cellular roles include promoting cell-to-cell and cell-to-substratum adhesion, increasing cell proliferation, and inducing cytokeratin hyper-expression.
• Known by multiple aliases including Dyskerin, NAP57, CBF5, and NOLA4; mutations in DKC1 are linked to dyskeratosis congenita, a multisystem disorder.
• Post-translational modifications such as acetylation and phosphorylation have been identified, likely regulating its function and interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–180 of human DKC1, which may include functionally important domains; cross-reactivity with mouse suggests sequence conservation between species.
• For Western blotting, expect a band at approximately 58 kDa; consider appropriate positive controls such as HeLa or HEK293 cell lysates.
• Given the ubiquitous expression, loading controls are essential for accurate quantification in WB.
• For immunohistochemistry and immunofluorescence, validate the antibody in your specific tissue or cell model to confirm localization to the nucleolus and Cajal bodies.
• The antibody may be used in ELISA; however, optimal dilution and blocking conditions should be experimentally determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams with reliable access to IVD-grade raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For ribosome biogenesis and telomere biology research, we offer curated products like this DKC1 antibody to streamline your assay development. Our global supply chain can also source rare-target reagents, supporting projects from concept to clinic.