Anti-DJ-1/PARK7 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q99497

Core Product Specifications and Parameters

Biological Background: DJ-1/PARK7 Function and Localization

• DJ-1/PARK7 is a multifunctional protein primarily known as Parkinson disease protein 7, also identified as Maillard deglycase, oncogene DJ1, and protein/nucleic acid deglycase DJ-1. It acts as a redox-sensitive chaperone and protease, playing a critical role in protecting cells against oxidative stress and cell death. Related references: PMID:12796482, PMID:17015834, PMID:18711745
• The protein stabilizes neuroprotective factors such as NFE2L2 and PINK1, positively regulates the androgen signaling pathway, and modulates NF-kappa-B signaling, thereby influencing cell growth, transformation, and male fertility. Related references: PMID:12612053, PMID:14749723, PMID:15502874
• DJ-1/PARK7 has been described as a deglycase that repairs methylglyoxal- and glyoxal-glycated proteins and nucleic acids, releasing lactate or glycolate and preventing advanced glycation endproduct (AGE) formation. However, this deglycase activity remains controversial and is disputed by other studies. Related references: PMID:25416785, PMID:28596309, PMID:26995087, PMID:28013050, PMID:27903648, PMID:31653696
• The protein protects histones from methylglyoxal adduction, thereby preserving local and global chromatin architecture and maintaining normal histone acetylation and ubiquitination levels. Related references: PMID:30150385, PMID:30894531
• DJ-1/PARK7 is essential for correct mitochondrial morphology and function; it regulates autophagy of dysfunctional mitochondria, attenuates oxidative stress in dopaminergic neurons, and modulates mitochondrial uncoupling proteins. Related references: PMID:16632486, PMID:19229105, PMID:18711745
• The protein exhibits a very low glyoxalase activity and directly eliminates hydrogen peroxide, providing protection against hydrogen peroxide-induced cell death. Related references: PMID:22523093, PMID:28993701, PMID:31653696, PMID:16390825
• DJ-1/PARK7 binds to specific mRNAs containing GG or CC motifs, partially inhibiting their translation; this interaction is disrupted under oxidative stress conditions. Related references: PMID:18626009
• The protein also functions as a metal-binding protein, interacting with copper and toxic mercury ions to enhance cellular protection against metal-induced toxicity. Related references: PMID:23792957
• The protein localizes to the cell membrane, cytoplasm, nucleus, membrane rafts, mitochondrion, and endoplasmic reticulum. It is highly expressed in pancreas, kidney, skeletal muscle, liver, testis, and heart, with lower levels in placenta and brain, including astrocytes and germ cells. Related references: PMID:22611253

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the N-terminal region (amino acids 1–100) of human DJ-1/PARK7, which typically yields antibodies recognizing the full-length protein.
• For Western blotting (WB), the predicted molecular weight is ~20 kDa; consider validating antibody specificity using relevant cell lines (e.g., HeLa, MCF7) that endogenously express DJ-1/PARK7.
• For immunohistochemistry on paraffin sections (IHC-P), antigen retrieval optimization may be required. Validate staining patterns in human, mouse, or rat tissues based on the antibody's species cross-reactivity.
• For ELISA-based assays, using a recombinant DJ-1/PARK7 protein standard is recommended for quantification and assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As a trusted sourcing partner, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your IVD development and production needs. Our DJ-1/PARK7 antibody is suitable for research on oxidative stress, neurodegeneration, and mitochondrial dysfunction, and we can assist with custom conjugation, kit development, and technical consultation.