Anti-DISP1 Rabbit Polyclonal Antibody for WB, ELISA - Q96F81

Core Product Specifications and Parameters

Biological Background: DISP1 Function and Localization

• Protein dispatched homolog 1 (DISP1), also known as DISPA, is a transmembrane protein encoded by the DISP1 gene in humans.
• It functions in the hedgehog (Hh) signaling pathway, regulating the release and extracellular accumulation of cholesterol-modified hedgehog proteins, which is essential for effective Hh signal production.
• DISP1 synergizes with SCUBE2 to enhance SHH (Sonic Hedgehog) secretion, as demonstrated in functional studies. Related references: PMID:22902404
• The protein localizes to the membrane, consistent with its role in cholesterol-modified protein secretion.
• DISP1 is annotated with keywords including developmental protein, disease variant, glycoprotein, and transmembrane helix, reflecting its roles in development and membrane association.
• Genetic variants in DISP1 have been linked to holoprosencephaly, a developmental disorder of the forebrain.

Experimental Guidance and Technical Tips

• This polyclonal antibody, raised against a recombinant fusion protein corresponding to amino acids 1325-1524 of human DISP1, is suitable for Western blot (WB) and ELISA applications on human samples.
• For WB, the predicted molecular weight is 171 kDa; consider using a low-percentage gel and extended transfer times to ensure efficient blotting of high-molecular-weight proteins.
• Due to the membrane localization of DISP1, use appropriate lysis buffers containing detergents to effectively extract the protein.
• For ELISA, optimize antigen coating and antibody detection conditions; the immunogen can serve as a positive control.
• Validate antibody specificity in your experimental system using appropriate controls, as cross-reactivity with other species has not been tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For hedgehog signaling research and developmental biology applications, we can source rare targets like DISP1 and provide customized raw-material solutions. Contact us to streamline your assay development from concept to clinic.