Anti-DISC1 Rabbit Polyclonal Antibody for WB and ELISA - Q9NRI5

Core Product Specifications and Parameters

Biological Background: DISC1 Function and Localization

• DISC1 is a scaffold protein crucial for embryonic and adult neurogenesis, integrating multiple signaling pathways.
• Positively regulates Wnt-mediated neural progenitor proliferation by modulating GSK3B and CTNNB1 stability. Related references: PMID:19303846
• Modulates AKT-mTOR signaling to control the tempo of newborn neuron integration, including dendritic development and synapse formation.
• Regulates hippocampal granule cell precursor migration during development. Related references: PMID:19502360
• Associates with PCNT to participate in microtubule network formation. Related references: PMID:18955030
• Localizes to multiple subcellular compartments, including cytoplasm, cytoskeleton, mitochondria, centrosome, and postsynaptic density.
• Ubiquitously expressed, with highest levels in the hippocampus; also present in temporal and parahippocampal cortices and white matter.
• Linked to schizophrenia susceptibility; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-200) of human DISC1, which is conserved across human, mouse, and rat, supporting the reported cross-reactivity.
• For western blotting, consider an initial dilution range of 1:500-1:2000 and expect a band around 94 kDa in appropriate cell or tissue lysates. Optimize based on your sample system.
• For ELISA, the antibody can be used for antigen capture or detection. Validate pairing with an appropriate monoclonal antibody if developing a sandwich assay.
• Due to the polyclonal nature, lot-to-lot consistency may be monitored by testing on reference cell lines expressing endogenous DISC1.
• Include appropriate positive and negative controls to confirm specificity in your experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop IVD raw-material sourcing partner, bridging diagnostic developers with high-quality reagents and technical support from concept to clinic. This anti-DISC1 polyclonal antibody, targeting a key neurodevelopmental protein, is part of our extensive portfolio ideal for neuroscience and psychiatric disorder research. Beyond catalog antibodies, we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist in custom rare-target raw material sourcing to meet your IVD assay development needs.