Anti-DIO3 Rabbit Polyclonal Antibody for WB & ELISA - P55073

Core Product Specifications and Parameters

Biological Background: DIO3 Function and Localization

• Protein identity: Thyroxine 5-deiodinase (D3, 5DIII, DIOIII) encoded by the DIO3 gene, also known by aliases TXDI3 and ITDI3.
• Core catalytic function: Catalyzes inner-ring deiodination (IRD) of thyroid hormones, converting the prohormone T4 to inactive reverse T3 (rT3) and active T3 to inactive 3,3'-T2, thereby regulating thyroid hormone action. Related references: PMID:7593630, PMID:12586771, PMID:12746313
• Additional outer-ring deiodination (ORD) activity: Catalyzes the deiodination of 3-monoiodothyronine (3-T1) to L-thyronine (T0). Related references: PMID:18821722
• Broad substrate scope: Also deiodinates iodothyronamines, including 3,3',5,5'-tetraiodothyronamine and others. Related references: PMID:18339710
• Subcellular localization: Localizes to the cell membrane and endosome membrane via a signal-anchor transmembrane domain.
• Tissue specificity: Expressed in placenta and several fetal tissues, underlining its role in developmental thyroid hormone regulation.
• Structural features: Contains selenocysteine (Sec) essential for catalytic activity; belongs to the deiodinase family of oxidoreductases; membrane-bound with a transmembrane helix.

Experimental Guidance and Technical Tips

• For WB analysis, DIO3 has a predicted molecular weight of approximately 34 kDa. However, post-translational modifications (e.g., glycosylation, membrane integration) may cause migration shifts. Validate the banding pattern in your specific cell/tissue lysate.
• As a rabbit polyclonal antibody, it recognizes multiple epitopes on the immunogen (residues 70-170), providing robust detection. Consider that lot-to-lot variability may occur; validate each new lot for consistency.
• For ELISA, the antibody can be used as a capture or detection reagent. Pair it with a matched monoclonal antibody for sandwich ELISA development, and optimize coating concentrations experimentally.
• The target protein is membrane-bound; consider using membrane protein extraction protocols (e.g., with mild detergents) to enhance solubilization and detection.
• Cross-reactivity is confirmed for human; for other species, check sequence homology of the immunogen region before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This DIO3 antibody supports research into thyroid hormone metabolism and related diagnostic development. Our comprehensive supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing—streamlining your path from discovery to diagnostic application.