Anti-DiMethyl-Histone H3 (Lys79) Rabbit mAb for Chromatin/Epigenetic Research (Q16695/P68431)

Core Product Specifications and Parameters

Biological Background: DiMethyl-Histone H3-K79 Function and Localization

• Histone H3 is a core nucleosome component, central to DNA packaging and regulation of transcription, replication, and repair. It undergoes extensive post-translational modifications, including methylation, to modulate chromatin structure and function. Related references: UniProt Q16695, UniProt P68431
• This antibody specifically detects histone H3 dimethylated at lysine 79 (H3K79me2), a conserved mark involved in epigenetic regulatory processes. The dimethyl form is often associated with active transcription and telomeric silencing. Related references: UniProt Q16695, UniProt P68431
• H3.1t (Q16695) is a testis-specific histone variant, whereas H3.1 (P68431) is ubiquitously expressed across tissues. Both localize to the nucleus and chromatin. Related references: UniProt Q16695, UniProt P68431

Experimental Guidance and Technical Tips

• For Western blotting, the expected molecular weight is ~15 kDa; use a high-percentage acrylamide gel (e.g., 15%) and optimize transfer conditions for low molecular weight proteins.
• Suitable for chromatin immunoprecipitation (ChIP and ChIP-seq) to map H3K79me2 distribution; consider cross-linking and sonication protocols specific to histone modifications.
• Validated for multiple immunofluorescence applications (IF/ICC, IF-P, IHC-P); titrate the antibody and use appropriate fixation/permeabilization methods.
• Cross-reactivity is predicted for human, mouse, rat, and other species; verify performance in your experimental system.
• The antibody can be used in ELISA and dot blot (DB) for quantitative detection of modified histone peptides.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing hub for IVD raw materials, offering diagnostic manufacturers, laboratories, and research institutes access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For epigenetic research and chromatin biology studies, CamelBio can facilitate procurement of this DiMethyl-Histone H3-K79 Rabbit mAb along with other rare-target antibodies and custom development services, streamlining your transition from concept to clinic.