Anti-DiMethyl-DNMT3A-K44 (O88508) Rabbit Polyclonal Antibody for IF/ICC and ELISA

Core Product Specifications and Parameters

Biological Background: DNA (cytosine-5)-methyltransferase 3A (DNMT3A) Function and Localization

• DNMT3A is a key enzyme required for genome-wide de novo DNA methylation, essential for establishing methylation patterns during development. It modifies DNA in a non-processive manner and can methylate both CpG and non-CpG sites.
• The enzyme functions in coordination with histone modifications, preferentially methylating linker DNA between nucleosomes and is inhibited by histone H1. It also plays a role in paternal and maternal imprinting, with methylation of most imprinted loci in germ cells.
• DNMT3A acts as a transcriptional corepressor, for example, by interacting with ZBTB18, and can repress transcription via recruitment of HDAC activity. It is recruited to genomic regions marked by trimethylated histone H3 at Lys36 (H3K36me3).
• Subcellularly, DNMT3A localizes predominantly in the nucleus and associates with chromosomes, accumulating at pericentric heterochromatin major satellite repeats. It may also be found in the cytoplasm.
• Two isoforms exist: isoform 1 is ubiquitously expressed at low levels, while isoform 2 expression is restricted to tissues undergoing active de novo methylation, such as spleen, testis, and thymus.
• The protein undergoes multiple post-translational modifications, including phosphorylation (e.g., at Ser102, Ser239, Ser251, Ser386, Ser389, Thr120, Thr257), methylation (e.g., omega-N-methylarginine at Arg167), and auto-methylation on Cys706.

Related references: PMID:10555141, PMID:11399089, PMID:11919202, PMID:16567415, PMID:17713477, PMID:9662389, PMID:11350943, PMID:18823905, PMID:15215868, PMID:20547484, PMID:21481189, PMID:12138111, PMID:15456878, PMID:21183079, PMID:24129315

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide dimethylated at Lys44 of mouse DNMT3A. For IF/ICC, consider that this modification may be context-specific; validate in your system by comparing signals between methylated and unmethylated peptide blocking or using knockout/knockdown controls.
• ELISA applications can benefit from using the immunizing peptide as a positive control and an unmodified peptide as a negative control to confirm dimethyl-specificity.
• Given the cross-reactivity with human and rat, this antibody may be suitable for studying conserved methylation sites across species, but confirm species-specific reactivity in your assays.
• Optimal working dilutions should be empirically determined; typical ranges for IF/ICC and ELISA are application-dependent and must be optimized by the end-user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing diagnostic manufacturers, laboratories, and research institutes with a seamless, one-stop procurement experience for high-quality IVD raw materials and technical services. This rabbit polyclonal antibody against dimethylated DNMT3A-K44 (O88508) exemplifies our commitment to supplying validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target-specific raw materials for epigenetic research. Whether you need off-the-shelf products or custom sourcing solutions, CamelBio is your trusted partner from concept to clinic.