Anti-DICER1 Polyclonal Antibody for IF/ICC, ELISA - Q9UPY3

Core Product Specifications and Parameters

Biological Background: DICER1 Function and Localization

• DICER1 encodes endoribonuclease Dicer, a large ~219 kDa protein belonging to the RNase III family.
• It cleaves double-stranded RNA and pre-miRNA into siRNA and miRNA, respectively, which guide the RNA-induced silencing complex (RISC) for gene silencing.
• Through miRNA processing, it regulates gene expression post-transcriptionally; through siRNA, it mediates RNA interference, controlling transcripts from mobile genetic elements and viral RNA.
• Subcellular localization is primarily cytoplasmic, with perinuclear enrichment.
• Contains helicase, RNase III, and dsRNA-binding domains; requires metal ions (Mg²⁺ or Mn²⁺) for activity; phosphorylated.
• Alternative splicing generates multiple isoforms; disease variants are associated with familial tumor predisposition syndromes.

Experimental Guidance and Technical Tips

• For IF/ICC, consider optimizing fixation and permeabilization protocols for cytoplasmic target detection.
• In ELISA, the peptide immunogen suggests good recognition of linear epitopes; validate performance with your specific sample matrix.
• As a polyclonal antibody, include appropriate controls such as pre-immune serum or blocking peptide to confirm specificity.
• Due to alternative splicing, the antibody may detect multiple isoforms; validate specificity in your system using knockdown or knockout controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials and technical services, supporting diagnostic manufacturers and researchers from concept to clinic. For RNA silencing and oncology research, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your assay development and supply chain.