Anti-DICER1 Monoclonal Antibody for WB, IHC-P, ELISA - Q9UPY3

Core Product Specifications and Parameters

Biological Background: DICER1 Function and Localization

• DICER1 is a ~219 kDa endoribonuclease belonging to the RNase III family, also known as Helicase with RNase motif.
• It plays a central role in RNA interference by cleaving long double-stranded RNA (dsRNA) into small interfering RNAs (siRNAs) and processing precursor microRNAs into mature miRNAs, each approximately 21-23 nucleotides long.
• These small RNAs are loaded into the RNA-induced silencing complex (RISC), guiding sequence-specific degradation or translational repression of target mRNAs.
• DICER1 localizes primarily to the cytoplasm, with enrichment in the perinuclear region.
• The protein contains conserved helicase and RNase III domains, as well as an ATP-binding site, and requires divalent metal ions such as magnesium or manganese for catalytic activity.
• Post-translational modifications, including phosphorylation, and alternative splicing generate multiple DICER1 isoforms, which may have distinct regulatory roles.
• Given its function, DICER1 is implicated in diverse biological processes including development, antiviral defense, and tumor suppression.

Experimental Guidance and Technical Tips

• For Western blot detection of the high-molecular-weight DICER1 protein (~219 kDa), use low-percentage acrylamide gels and extended transfer times with high-molecular-weight cut-off membranes.
• The recombinant fragment immunogen may cross-react with DICER1 isoforms; validate specificity in your experimental system using appropriate controls.
• For immunohistochemistry (IHC-P), antigen retrieval methods such as heat-induced epitope retrieval (HIER) in citrate or EDTA buffer may improve staining; optimize detection conditions for your tissue type.
• When performing ELISA, titrate the antibody to determine optimal coating and detection concentrations, and include species-matched secondary antibodies for reliable signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This DICER1 monoclonal antibody is part of our extensive portfolio supporting RNA biology and gene silencing research, available alongside validated antibody pairs, recombinant proteins, and ancillary reagents. For rare-target sourcing or custom assay development, our team bridges the gap between research needs and reliable commercial supply.