Anti-DIAPH3 Polyclonal Antibody for WB, ELISA - Q9NSV4

Core Product Specifications and Parameters

Biological Background: DIAPH3 Function and Localization

• DIAPH3, also known as diaphanous-related formin-3 (DRF3), mDia2, or DIA2, is a member of the formin family of actin nucleation and elongation factors.
• It is essential for the assembly of F-actin structures such as actin cables and stress fibers, playing a critical role in cytokinesis and transcriptional activation of serum response factor (SRF).
• DIAPH3 functions downstream of Rho GTPase signaling, binding to the GTP-bound form of Rho and recruiting profilin to the membrane to promote actin polymerization. This couples Rho and Src tyrosine kinase in signaling.
• In the nucleus, DIAPH3 promotes actin polymerization to drive serum-dependent SRF-MRTFA transcriptional activity, linking cytoskeletal dynamics to gene expression.
• DIAPH3 localizes to both the cytoplasm and the nucleus, consistent with its dual roles in actin organization and transcriptional regulation.
• It is subject to post-translational modifications including phosphorylation and ubiquitin-like conjugation, and alternative splicing generates multiple isoforms.
• Mutations in DIAPH3 are associated with autosomal dominant nonsyndromic hearing loss (AUNA1) and neuropathy, reflecting its importance in auditory and nervous system function.

Experimental Guidance and Technical Tips

• For Western blot, consider using a whole cell lysate from a human cell line (e.g., HeLa) and validate with molecular weight markers; the predicted molecular weight is around 137 kDa, but bands may shift due to phosphorylation.
• For ELISA, the antibody may be suitable for detecting recombinant DIAPH3 protein; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing. This anti-DIAPH3 polyclonal antibody supports research in actin dynamics, cytoskeletal organization, and hearing loss studies.