Anti-DIAPH2 Rabbit Polyclonal Antibody for WB, ELISA - O60879

Core Product Specifications and Parameters

Biological Background: DIAPH2 Function and Localization

• DIAPH2, also known as Diaphanous-related formin-2 (DRF2) or DIA2, is a member of the formin protein family involved in actin cytoskeleton organization.
• The protein is implicated in oogenesis, with the gene being identified as a candidate for premature ovarian failure (POF), reflected in its aliases POF, POF2, and POF2A.
• It plays a role in endosome dynamics, regulating the motility of early endosomes through a signal transduction pathway involving isoform 3, CSK, and RHOD.
• DIAPH2 is expressed in multiple tissues, including testis, ovary, small intestine, prostate, lung, liver, kidney, and leukocytes.
• At the subcellular level, DIAPH2 localizes to the cytoplasm and cytosol, and is found on early endosomes.
• The protein is subject to acetylation and alternative splicing, yielding multiple isoforms (e.g., isoform 3) with potentially distinct functions.
• Keywords associated with DIAPH2 include Coiled coil, Developmental protein, Differentiation, making it a focus in developmental and cell signaling research.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal 120 amino acids (aa 1-120) of human DIAPH2. Verify reactivity with endogenous protein and specific isoforms in your experimental system.
• For Western blotting, reduce and denature samples prior to loading, and use a positive control such as testis or ovary tissue lysate where DIAPH2 is known to be expressed.
• Optimal antibody dilution for each application should be determined empirically. Consider using purified recombinant DIAPH2 protein as a standard for ELISA.
• Due to potential isoform diversity, confirm detection specificity with siRNA knockdown or isoform-specific controls where feasible.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop solution for IVD raw materials, serving diagnostic manufacturers and research laboratories with a focus on developmental and cell biology targets. For DIAPH2-related studies, we provide a range of polyclonal and monoclonal antibodies, validated antibody pairs, and bulk ancillary reagents to support your assay development and production needs. Our dedicated sourcing team can also assist with rare targets and customized reagent procurement, ensuring a seamless transition from concept to clinic.