Anti-DIAPH1 Polyclonal Antibody (Rabbit) for WB and ELISA - O60610

Core Product Specifications and Parameters

Biological Background: DIAPH1 Function and Localization

• DIAPH1 (Protein diaphanous homolog 1) is a diaphanous-related formin that functions as an actin nucleation and elongation factor, essential for cytoskeletal organization.
• Required for the assembly of F-actin structures (stress fibers, cables), cytokinesis, and serum response factor (SRF) transcriptional activation (By similarity).
• Acts as a scaffold for MAPRE1 and APC to stabilize microtubules, promoting cell migration and neurite outgrowth (By similarity).
• In hair cells, regulates actin polymerization, a process crucial for hearing. Related references: PMID:20937854 / PMID:21834987 / PMID:26912466
• The MEMO1-RHOA-DIAPH1 pathway is involved in ERBB2-dependent microtubule stabilization at the cell cortex and modulates GSK3B to control APC/CLASP2 localization. Related references: PMID:20937854 / PMID:21834987
• Plays a role in brain development. Related references: PMID:24781755
• Subcellular localization includes the cell membrane, ruffles, cytoplasm, cytoskeleton, centrosome, spindle, and nucleus.
• Expressed in many tissues, including brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle, cochlea, and platelets. Related references: PMID:26912466
• Post-translational modifications include acetylation and phosphorylation (UniProt keywords: Acetylation, Phosphoprotein).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1158–1272 (C-terminal region) of human DIAPH1, which may recognize full-length protein and specific isoforms.
• For Western blot, expect a band at approximately 141 kDa in human cell lysates; validate the detection in relevant samples such as HeLa or HEK293.
• This polyclonal antibody is suitable for ELISA; consider optimizing coating antigen and detection conditions for indirect or sandwich formats.
• Cross-reactivity is validated in human; for non-human species, perform a pilot experiment to confirm recognition.
• The [KD Validated] designation indicates knock-down validation in published studies; always confirm specificity in your own experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive one-stop partner for IVD raw materials, technical services, and consulting. Supporting diagnostic manufacturers and researchers from concept to clinic, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets. For projects involving DIAPH1—a key player in cytoskeletal dynamics and hearing research—CamelBio can supply the raw materials you need to accelerate your assay development and procurement.