Anti-DHX9/RNA Helicase A Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q08211

Core Product Specifications and Parameters

Biological Background: DHX9/RNA Helicase A Function and Localization

• DHX9, also known as RNA helicase A, DEAH box protein 9, leukophysin (LKP), and nuclear DNA helicase II (NDH II), is a multifunctional DEAH-box RNA helicase.
• Possesses ATP-dependent 3'→5' helicase activity unwinding a broad range of substrates including dsDNA, dsRNA, DNA:RNA hybrids, forks, D-loops, R-loops, triplex DNA, and G-quadruplexes; requires a 3' single-stranded tail for entry. Binds ssDNA, ssRNA, poly(A) RNA, and circular substrates; stimulates TOP2A-mediated supercoil relaxation. Related references: PMID:1537828 PMID:20669935 PMID:21561811 PMID:24049074 PMID:12711669
• Acts as a transcriptional coactivator bridging RNA polymerase II with transcription factors (BRCA1, CREBBP, RELA, SMN1) and binds the CDKN2A promoter. Related references: PMID:11038348 PMID:11149922 PMID:11416126
• Plays diverse roles in post-transcriptional gene regulation: cooperates with NUP98 in alternative splicing, resolves harmful inverted Alu-repeat dsRNA structures, and facilitates nuclear export of CTE-containing unspliced mRNAs. Related references: PMID:11402034 PMID:16680162 PMID:28221134 PMID:28355180 PMID:10924507 PMID:9162007
• Involved in mRNA translation: enhances translation via PCE in 5'-UTR, stabilizes MYC mRNA through the CRD-mediated complex, and stimulates collagen and LIN28A-dependent translation together with LARP6 and LIN28A. Related references: PMID:16680162 PMID:19029303 PMID:22190748 PMID:21247876
• Functions as an siRNA-loading factor for RISC assembly and RNA-mediated gene silencing. Related references: PMID:17531811
• In innate immunity, binds short rotavirus-derived dsRNA and triggers NLRP9 inflammasome activation, leading to IL-18 release and pyroptosis. Related references: PMID:28636595
• Predominantly nuclear (nucleoplasm, nucleolus) but also localizes to the cytoplasm, centrosome, and cytoskeleton; subject to extensive post-translational modifications including phosphorylation, acetylation, methylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 329–563 of DHX9, a region conserved across human, mouse, and rat; this sequence lies within the helicase core domain and is expected to detect multiple isoforms.
• For Western blotting, the predicted molecular weight is ~141 kDa. Ensure adequate protein transfer and use appropriate molecular weight markers. Optimize blocking and antibody dilution for your sample type.
• For immunofluorescence/immunocytochemistry, DHX9 is distributed in both nuclear and cytoplasmic compartments. Validate fixation/permeabilization conditions to preserve antigen accessibility and consider using co-stains for subcellular structures.
• For immunoprecipitation, employ optimized lysis buffers containing protease and phosphatase inhibitors to maintain protein interactions. A negative control (e.g., rabbit IgG) is essential.
• ELISA performance should be calibrated with purified antigen or cell lysates, and cross-reactivity with related DEAH-box helicases may be evaluated depending on experimental specificity requirements.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and academic institutions with comprehensive access to IVD raw materials and technical support across the entire development workflow. This anti-DHX9 polyclonal antibody is well-suited for investigators studying transcriptional regulation, RNA biology, viral-host interactions, and innate immunity. Beyond single-antibody procurement, CamelBio offers validated antibody pairs, monoclonal alternatives, bulk ancillary reagents, and customized sourcing for rare-target raw materials—serving as a reliable supply partner to streamline your research and assay development.