Anti-DHX8 Rabbit Polyclonal Antibody for WB, ELISA - Q14562

Core Product Specifications and Parameters

Biological Background: DHX8 Function and Localization

• DHX8 is a DEAH-box RNA helicase and an essential component of the spliceosome, where it participates in pre-mRNA splicing (PubMed:11991638, PubMed:28076346, PubMed:28502770). Related references: PMID:11991638 PMID:28076346 PMID:28502770
• It facilitates nuclear export of spliced mRNA by releasing the mature transcript from the spliceosome (PubMed:8608946). Related references: PMID:8608946
• DHX8 is localized to the nucleus.
• The protein is expressed in skin, cervix, nerve, and brain.
• It belongs to the DEAH-box helicase family and contains ATP-binding and helicase domains.
• Post-translational modifications include phosphorylation and ubiquitination (isopeptide bond and Ubl conjugation).

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (amino acids 1001–1220) of human DHX8, which may aid in recognizing the full-length protein as well as potential C-terminal fragments.
• For Western blot, consider using a positive control lysate from human cell lines known to express DHX8 (e.g., HeLa or neuronal cells); the expected molecular weight is approximately 139 kDa.
• Validate the antibody for ELISA using recombinant DHX8 protein or peptide coating antigens; note that cross-reactivity with other DEAH-box proteins has not been determined.
• Optimize blocking and washing conditions to reduce background, as polyclonal antibodies can exhibit varied reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized one-stop provider of IVD raw materials, offering comprehensive support from research concept to clinical application. This anti-DHX8 polyclonal antibody supports studies of mRNA splicing and gene expression, and CamelBio can source validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for your diagnostic development. Our team is ready to assist with rare target raw-material procurement to accelerate your assay workflow.