Anti-DHX58 Polyclonal Antibody for WB, IF/ICC, ELISA - Q96C10

Core Product Specifications and Parameters

Biological Background: DHX58 Function and Localization

• DHX58, also known as LGP2 or RLR-3, is an ATP-dependent RNA helicase and a member of the RIG-I-like receptor (RLR) family. It lacks the CARD domain required for direct MAVS activation, serving as a regulator rather than an initiator of antiviral signaling.
• DHX58 exerts dual regulatory roles on RIG-I and MDA5 signaling: it can negatively regulate by competing for viral RNA or binding to RIGI and preventing its interaction with MAVS, and positively regulate by unwinding viral nucleoproteins to facilitate their recognition by RIG-I and MDA5.
• It is involved in the innate immune response to a broad range of pathogens, including RNA viruses, DNA viruses (such as poxviruses and SARS-CoV-2), and the bacterium Listeria monocytogenes. Related references: PMID:31256877
• DHX58 binds both single-stranded RNA (ssRNA) and double-stranded RNA (dsRNA) with higher affinity for dsRNA, and shows a preference for 5'-triphosphorylated RNA.
• Subcellularly, DHX58 localizes predominantly to the cytoplasm.
• Tissue expression analysis shows DHX58 in testis, nerve, spleen, and brain.
• Key structural and functional features include ATP-binding, helicase activity, zinc-binding, and involvement in antiviral defense and innate immunity.

Experimental Guidance and Technical Tips

• The immunogen used for this polyclonal antibody corresponds to amino acids 389-678 of human DHX58. When performing Western blot, expect a band at approximately 77 kDa; validate the antibody using relevant cell lysates expressing DHX58.
• For immunofluorescence/ICC, anticipate cytoplasmic staining patterns. Confirm specificity by comparing with negative controls and known localization.
• This antibody cross-reacts with mouse and rat DHX58, making it suitable for studies in these model organisms. However, validation in the specific sample system is recommended.
• In ELISA applications, consider pairing with a detection antibody targeting a non-overlapping epitope. Optimize coating and detection conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, offering diagnostic developers and research labs one-stop access to validated antibodies, antigen, and ancillary reagents. For innate immunity and antiviral signaling targets like DHX58, CamelBio provides a broad supply capability—from optimized polyclonal and monoclonal antibodies to bulk reagents and rare target sourcing—to accelerate your assay development from concept to clinic.