Anti-DHX57 Polyclonal Antibody for WB and ELISA - Q6P158

Core Product Specifications and Parameters

Biological Background: DHX57 Function and Localization

• DHX57, also known as DDX57 or DEAH box protein 57, is a putative ATP-dependent RNA helicase belonging to the DEAH-box helicase family.
• The protein contains characteristic helicase domains, including an ATP-binding domain, and is predicted to utilize ATP hydrolysis to unwind RNA secondary structures.
• Alternative splicing of the DHX57 gene yields multiple isoforms (indicated by the "Alternative splicing" keyword); the immunogen sequence corresponds to a region common to described isoforms.
• DHX57 features a coiled-coil domain and a zinc-finger motif, suggesting roles in protein-protein or protein-nucleic acid interactions (as per keywords: Coiled coil, Zinc, Zinc-finger).
• The protein is subject to phosphorylation (Phosphoprotein keyword), indicating potential regulation via kinase signaling pathways.
• Molecular weight is approximately 156 kDa (observed at ~155.6 kDa by sequence), consistent with the 156kDa specification.
• Limited information on subcellular localization; as an RNA helicase, it is likely involved in nuclear or cytoplasmic RNA metabolism.
• No tissue-specific expression data available; the protein has been identified in proteomics studies (Reference proteome, Proteomics identification keywords).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment covering amino acids 700-900, which lies within a conserved region of DHX57; this may contribute to cross-reactivity with mouse.
• For Western blot, consider using whole cell lysates from human or mouse cell lines known to express DHX57, and validate band size (~156 kDa) using appropriate molecular weight markers.
• ELISA application suggests suitability for detecting native or recombinant DHX57; optimize coating and detection conditions in the relevant sample matrix.
• Always include proper positive and negative controls (e.g., knockdown lysates or recombinant protein) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop provider of IVD raw materials, offering validated antibodies, bulk ancillary reagents, and rare target sourcing to diagnostic manufacturers and research labs worldwide. The DHX57 polyclonal antibody supports investigations into DEAH-box RNA helicases and ATP-dependent unwinding mechanisms. Contact us for customized supply solutions, including antibody pairs and reagent scaling, from concept to clinic.