Anti-DHX38 Rabbit Polyclonal Antibody for WB, ELISA - Q92620

Core Product Specifications and Parameters

Biological Background: DHX38 Function and Localization

• DHX38 (DEAH box protein 38), also known as PRP16, DDX38, or PRPF16, is an ATP-dependent RNA helicase belonging to the DEAH-box family of helicases.
• It is a probable ATP-binding RNA helicase that plays a key role in pre-mRNA splicing by functioning as a spliceosome component (Probable).
• DHX38 is essential for pre-mRNA splicing, participating in spliceosome assembly and catalytic activation (PubMed:29301961, PubMed:9524131). Related references: PMID:29301961 PMID:9524131
• The protein localizes predominantly to the nucleus, consistent with its involvement in mRNA processing.
• Post-translational modifications of DHX38 include acetylation, phosphorylation, and ubiquitin-like conjugation, which may regulate its function and interactions.
• Alternative splicing of DHX38 generates multiple isoforms, potentially contributing to functional diversity.
• Mutations in DHX38 are associated with retinitis pigmentosa, highlighting its significance beyond the spliceosome.

Experimental Guidance and Technical Tips

• For Western blotting, a band around 141 kDa is expected. Use appropriate nuclear protein extracts and validate with relevant positive and negative controls.
• Optimize antibody dilutions empirically for both WB and ELISA applications; typical starting ranges can be guided by general polyclonal antibody protocols.
• Confirm cross-reactivity in mouse and rat samples before large-scale experiments, as reactivity may vary across species.
• Consider using a loading control of similar molecular weight or probing membranes cautiously to avoid size-dependent transfer issues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing comprehensive IVD raw-material solutions, connecting diagnostic manufacturers and researchers with high-quality antibodies and ancillary reagents. For your splicing and RNA helicase research needs, including DHX38, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing assistance for rare targets. Leverage our integrated supply platform to accelerate your assay development from concept to clinic.