Anti-DHX36 Polyclonal Antibody for WB, IP, ELISA - Q9H2U1

Core Product Specifications and Parameters

Biological Background: DHX36 Function and Localization

• DHX36, also known as G4R1 or RHAU, is a multifunctional ATP-dependent DEAH-box helicase that specifically unwinds G-quadruplex (G4) structures in both DNA and RNA. Related references: PMID:16150737 PMID:18842585 PMID:20472641

• It resolves G4-DNA structures in gene promoters (e.g., YY1, KIT/c-kit) to positively regulate transcription, and processes G4 structures in telomeric RNA to maintain telomere integrity. Related references: PMID:21993297 PMID:22238380 PMID:20472641 PMID:21846770

• In post-transcriptional regulation, DHX36 unwinds G4-RNA in 3′-UTRs (e.g., TP53 pre-mRNA) to promote 3′-end processing, and modulates mRNA translation and stability by binding to AU-rich elements (AREs) and G4 structures. Related references: PMID:27940037 PMID:24369427 PMID:26489465 PMID:14731398 PMID:18279852

• DHX36 is involved in cytoplasmic mRNA decay and exosome-mediated degradation, and is a component of an antiviral dsRNA-sensing complex. Related references: PMID:14731398 PMID:18279852

• DHX36 localizes to multiple cellular compartments, including the nucleus, cytoplasm, stress granules, nuclear speckles, telomeres, mitochondria, and neuronal dendrites/axons.

• Highly expressed in testis; plays roles in development, hematopoiesis, spermatogonia differentiation, and heart development.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 1-260 amino acids of human DHX36. For Western blot, a band around 115 kDa is expected; consider using a reducing agent and appropriate lysis buffer to solubilize this nuclear/cytoplasmic protein.
• DHX36 expression may vary across tissues; validate antibody performance in your target sample system and include controls (e.g., DHX36 overexpression or knockdown).
• For immunoprecipitation, standard protocols with protein A/G beads are recommended; the antibody can capture endogenous DHX36 and associated complexes.
• This antibody is suitable for ELISA; optimize coating antigen and antibody dilutions for your specific assay format.
• Cross-reactivity with mouse and rat DHX36 is reported; verify sequence homology if using in other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering a curated portfolio of validated antibodies, including anti-DHX36 polyclonal antibodies. Whether your research focuses on G-quadruplex biology, RNA metabolism, or telomere maintenance, CamelBio streamlines procurement from discovery to clinical application. Our supply capabilities include antibody pairs, bulk reagents, and rare target sourcing to accelerate your diagnostic development.