Anti-DHX30 Rabbit Polyclonal Antibody for WB, ELISA - Q7L2E3

Core Product Specifications and Parameters

Biological Background: DHX30 Function and Localization

• DHX30, also known as DEAH box protein 30, is an ATP-dependent RNA helicase that belongs to the DEAH-box family and is implicated in ribosome biogenesis and RNA metabolism.
• It functions as an RNA-dependent helicase that uses the energy from ATP hydrolysis to unwind RNA substrates (PubMed:29100085). Related references: PMID:29100085
• DHX30 plays a critical role in the assembly of the mitochondrial large ribosomal subunit, which is essential for proper mitochondrial protein synthesis (PubMed:25683715, PubMed:29100085). Related references: PMID:25683715 PMID:29100085
• The protein directly associates with mitochondrial DNA within the mitochondrial nucleoid, indicating a potential role in mtDNA maintenance or expression (PubMed:18063578). Related references: PMID:18063578
• DHX30 is required for the optimal function of the zinc-finger antiviral protein ZC3HAV1, suggesting an involvement in the innate immune response against viral infections (By similarity).
• It contributes to nervous system development and differentiation by up-regulating key neurogenesis genes, including GSC, NCAM1, neurogenin, and NEUROD (By similarity).
• Subcellular localization studies reveal that DHX30 is primarily found in the mitochondrial matrix and nucleoid, with additional cytoplasmic distribution.
• Additional features include phosphorylation, multiple splice variants, and an association with intellectual disability when mutated (disease variant), highlighting its importance in neuronal function.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 906–1155) of human DHX30, which may facilitate recognition of full-length endogenous DHX30 across species. Researchers should validate reactivity in their specific sample types.
• For Western blotting, a major band at ~134 kDa is anticipated. Including mitochondrial-enriched lysates from human, mouse, or rat tissues may serve as robust positive controls.
• This antibody is also suitable for ELISA applications; optimal coating conditions and antibody dilutions should be determined empirically for each assay format.
• Consider pairing this polyclonal antibody with a monoclonal counterpart in sandwich ELISA development for increased specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio accelerates diagnostic development by providing a one-stop supply of high-quality IVD raw materials, including antibodies, antigens, and ancillary reagents. This anti-DHX30 antibody supports research in mitochondrial biology, neurodevelopment, and antiviral immunity, and CamelBio can fulfill bulk orders, source matched pairs, or procure rare target materials to streamline your assay pipeline. Partner with CamelBio to bridge from concept to clinic efficiently.