Anti-DHRS9 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BPW9

Core Product Specifications and Parameters

Biological Background: DHRS9 Function and Localization

• DHRS9, also known as dehydrogenase/reductase SDR family member 9, is a member of the short-chain dehydrogenase/reductase (SDR) family. It is encoded by the DHRS9 gene (also named RDH15, SDR9C4) in humans, and alternative splicing generates multiple transcript variants.
• It functions as a 3-alpha-hydroxysteroid dehydrogenase that converts 3-alpha-tetrahydroprogesterone (allopregnanolone) to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone, playing a key role in steroid hormone metabolism. Related references: PMID:11294878 PMID:29541409
• DHRS9 also participates in the biosynthesis of retinoic acid by catalyzing the oxidation of retinaldehyde to retinoic acid, contributing to retinoid signaling pathways. Related references: PMID:11304534 PMID:12618084
• The enzyme can utilize both NADH and NADPH as cofactors, demonstrating flexibility in its redox reactions.
• Subcellularly, DHRS9 is localized to the membrane of the endoplasmic reticulum and microsomes, indicating its involvement in membrane-associated metabolic processes.
• Tissue expression analysis reveals high levels in trachea and epidermis, with lower detection in spinal cord, bone marrow, brain, tongue, esophagus, heart, colon, testis, placenta, lung, skeletal muscle, and lymph node.
• The protein is annotated with keywords including Alternative splicing, Endoplasmic reticulum, Lipid metabolism, Membrane, Microsome, NAD, NADP, Oxidoreductase, and Steroid metabolism, reflecting its diverse functional associations.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein spanning amino acids 18-319 of human DHRS9, which covers the majority of the protein sequence and is expected to generate antibodies recognizing multiple epitopes.
• For Western blotting (WB), a band around 35 kDa is anticipated. Validate in the relevant sample system to confirm specificity and sensitivity.
• In immunofluorescence/immunocytochemistry (IF/ICC), consider using appropriate fixation and permeabilization protocols to detect the endoplasmic reticulum/microsomal membrane localization.
• ELISA applications may benefit from optimizing coating antigen and antibody concentrations for the specific assay format.
• Cross-reactivity is reported for human, mouse, and rat; for other species, verify sequence homology before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement to support your assay development from concept to clinic. For research into retinol and steroid metabolism, we can supply DHRS9 antibodies and related reagents to accelerate your diagnostic or life science projects. Contact us to discuss your specific sourcing needs.