Anti-DHRS2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q13268

Core Product Specifications and Parameters

Biological Background: DHRS2 Function and Localization

• DHRS2, also known as HEP27 or SDR25C1, is a member of the short chain dehydrogenase/reductase (SDR) family. It is encoded by the DHRS2 gene, with alternative splicing producing multiple transcript variants.
• NADPH-dependent oxidoreductase that reduces dicarbonyl compounds, playing a role in inactivation of reactive carbonyls. Shows reductase activity with substrates like 3,4-hexanedione, 2,3-heptanedione, and 1-phenyl-1,2-propanedione. Related references: PMID:16685466
• Exhibits minor hydroxysteroid dehydrogenase activity toward bile acids, but unlikely to control hormone levels. Does not act on retinoids or sugars. Related references: PMID:16685466
• Attenuates MDM2-mediated degradation of p53/TP53, leading to p53 stabilization and increased transcription activity, resulting in accumulation of MDM2 and CDKN1A/p21. This suggests a tumor-suppressive role. Related references: PMID:20547751
• Reduces proliferation, migration, invasion, and ROS production in cancer cells. Related references: PMID:29106393
• Subcellular localization includes the mitochondrial matrix and the nucleus, as per UniProt annotation.
• Widely expressed, with highest levels in liver and kidney; also detected in heart, spleen, skeletal muscle, placenta, and dendritic cells, but not in many other immune cells.
• Post-translational modifications include acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• For Western blotting, the antibody targets a ~30 kDa band. Validate specificity in DHRS2-expressing tissues such as liver or kidney, and consider using positive controls (e.g., HeLa or HepG2 cell lysates).
• In immunofluorescence/ICC, the antibody may label mitochondria and nuclei. Optimize fixation and permeabilization conditions to preserve epitope accessibility.
• For ELISA applications, the polyclonal nature may enhance detection sensitivity. Pair with a monoclonal detection antibody if developing a sandwich assay.
• Cross-reactivity with mouse and rat homologs is indicated; verify sequence homology before applying to these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw-material sourcing, supporting diagnostic manufacturers and research labs from early-stage development to clinical application. For DHRS2-related research in cancer biology, oxidative stress, and metabolic disorders, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our sourcing services also cover rare targets, ensuring you can secure the materials needed for reliable assay development.