Anti-DHRS11 Rabbit Polyclonal Antibody for WB and ELISA - Q6UWP2

Core Product Specifications and Parameters

Biological Background: DHRS11 Function and Localization

• DHRS11 is a short-chain dehydrogenase/reductase (SDR) family member encoded by the DHRS11 gene (also known as SDR24C1), with multiple isoforms generated through alternative splicing.
• It catalyzes the conversion of 17-keto groups of estrone, 4- and 5-androstenes, and 5-alpha-androstanes to 17-beta-hydroxyl metabolites, and reduces 3-keto groups of 3-, 3,17-, and 3,20-diketosteroids to 3beta-hydroxyl metabolites. Related references: PMID:26920053
• The enzyme converts the adrenal androgen precursors 11-keto-4-androstene-3,17-dione (11KA4) and 11-keto-5alpha-androstane-3,17-dione (11K-Adione) into the potent androgens 11-ketotestosterone (11KT) and 11-ketodihydrotestosterone (11KDHT), respectively. Related references: PMID:31926269
• DHRS11 may also reduce endogenous and exogenous alpha-dicarbonyl compounds and xenobiotic alicyclic ketones. Related references: PMID:26920053
• The protein is predicted to be secreted, supported by a signal peptide and UniProt annotation as Secreted.
• Isoform 1 is ubiquitously expressed with highest levels in testis, small intestine, colon, kidney, brain, and heart; Isoform 3 is expressed in brain, heart, and skeletal muscle.
• As an oxidoreductase, DHRS11 binds NADP as a cofactor and participates in lipid and steroid metabolic processes.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 1-260 of human DHRS11, covering the near full-length protein; perform appropriate positive and negative controls in your assay.
• For Western blot, the predicted molecular weight is approximately 28 kDa (UniProt: 28308 Da). Consider using reducing conditions and validate the antibody in relevant human or mouse tissue/cell lysates.
• This antibody is validated for ELISA and may be suitable for quantifying DHRS11 in biological fluids or cell culture supernatants, given its secreted nature.
• Cross-reactivity with mouse allows its use in mouse model studies; however, we recommend confirming specificity in the Mouse tissue background.
• Since DHRS11 has multiple isoforms, verify the detection of your isoform of interest, as the antibody’s epitope may recognize shared regions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, supporting your research from concept to clinic. This anti-DHRS11 antibody is part of our expanding portfolio for steroid metabolism and hormone-related assays, and we also offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare or custom targets, our team can assist in sourcing and development to meet your specific needs.