Anti-DHPS Rabbit Polyclonal Antibody for WB and ELISA - P49366

Core Product Specifications and Parameters

Biological Background: Deoxyhypusine synthase (DHPS) Function and Localization

• DHPS (deoxyhypusine synthase) is a transferase that catalyzes the initial step of hypusine biogenesis: the NAD-dependent cleavage of spermidine and transfer of its butylamine moiety to a specific lysine residue of the eIF-5A precursor protein, forming deoxyhypusine (PubMed:30661771). Related references: PMID:30661771
• This post-translational hypusination is unique and essential for the function of mature eIF-5A, a factor implicated in translation elongation, mRNA turnover, and cell proliferation.
• Encoded by the DHPS gene (also known as DS, DHS, MIG13, NEDSSWI), the protein has a calculated molecular weight of approximately 41 kDa.
• Alternative splicing and phosphorylation are indicated; mutations in DHPS have been associated with neurodevelopmental disorders such as intellectual disability.
• The immunogen comprises a recombinant fusion protein spanning the full-length human DHPS (amino acids 1-369), ensuring broad epitope coverage.

Experimental Guidance and Technical Tips

• For Western blotting, the expected major band is observed at ~41 kDa; additional bands corresponding to alternative isoforms may be detected. Validate band specificity using knockout or knock-down controls.
• The polyclonal nature of this antibody makes it suitable for signal amplification in ELISA; consider paired detection approaches for quantitation.
• Cross-reacts with mouse and rat DHPS; verify signal in relevant tissue or cell lysates from these species before quantitative studies.
• Optimize antibody dilution and blocking conditions for your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering a one-stop platform for antibodies, proteins, and ancillary reagents. This anti-DHPS polyclonal antibody supports research into protein hypusination and translation control, and CamelBio’s sourcing capabilities extend to validated antibody pairs, recombinant proteins, and custom formulation for diagnostic development. From early assay design to clinical application, CamelBio bridges the gap between concept and clinic with reliable supply and technical guidance.