Anti-DHODH Rabbit Polyclonal Antibody for WB, ELISA - O35435

Core Product Specifications and Parameters

Biological Background: DHODH Function and Localization

• DHODH (dihydroorotate dehydrogenase (quinone), mitochondrial), also known as dihydroorotate oxidase, is a key enzyme in pyrimidine metabolism.
• It catalyzes the oxidation of dihydroorotate to orotate using quinone as an electron acceptor, an essential step in the de novo biosynthesis of uridine monophosphate (UMP).
• This reaction links the pyrimidine biosynthetic pathway to the mitochondrial electron transport chain, underscoring its role in cellular nucleotide balance.
• DHODH is localized to the mitochondrial inner membrane, consistent with its function in a membrane-associated process.
• The protein functions as a flavoprotein, utilizing flavin mononucleotide (FMN) as a cofactor and exhibiting oxidoreductase activity.
• Its critical role in UMP production makes it important for rapidly proliferating cells and a potential target for therapeutic intervention in certain diseases.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 77–395 of mouse DHODH, which likely targets the central region of the protein. Consider verifying antibody specificity using recombinant protein or biological samples expressing the target.
• For Western blot analysis, a band around 43 kDa is expected; appropriate loading controls and molecular weight markers are recommended.
• In ELISA applications, optimization of coating antigen concentration and antibody dilution may be required for optimal signal-to-noise ratios.
• Cross-reactivity with human DHODH is indicated; however, users should independently validate performance in their specific sample matrix and experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research labs, and institutions with streamlined access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our DHODH Rabbit pAb supports critical research into pyrimidine metabolism and mitochondrial function, reflecting our commitment to high-quality immunoreagents. From rare target sourcing to commercial-scale supply, CamelBio delivers end-to-end solutions tailored to your diagnostic development needs.