Anti-DHODH Polyclonal Antibody for WB, IHC-P, ELISA - Q02127

Core Product Specifications and Parameters

Biological Background: DHODH Function and Localization

• DHODH (dihydroorotate dehydrogenase (quinone), mitochondrial) is also known as dihydroorotate oxidase, an enzyme critical for the de novo biosynthesis of pyrimidines.
• It catalyzes the oxidation of dihydroorotate to orotate using quinone as an electron acceptor, a rate-limiting step in UMP production.
• The protein is localized to the mitochondrial inner membrane, where it functions as a flavoprotein with FMN as a cofactor.
• DHODH encodes a 43 kDa transmembrane protein that belongs to the dihydroorotate dehydrogenase family, containing a transit peptide and transmembrane helices for mitochondrial membrane insertion.
• As an oxidoreductase, it plays a vital role in nucleotide metabolism and cell proliferation, linking its activity to rapidly dividing cells and immune responses.
• Keywords from UniProt include disease variant, flavoprotein, FMN, mitochondrion inner membrane, and pyrimidine biosynthesis, highlighting its biomedical relevance.
• The immunogen used for this antibody spans amino acids 116-395, a region encompassing the catalytic domain, ensuring detection of endogenous DHODH.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved region of human DHODH (aa 116-395), suggesting the antibody may recognize mouse and rat orthologs, as indicated by cross-reactivity data.
• For Western blotting (WB), the predicted molecular weight is 43 kDa; using a reducing sample buffer and appropriate gel percentage is recommended.
• In immunohistochemistry (IHC-P), antigen retrieval protocols and tissue fixation methods should be optimized as DHODH is a membrane-bound mitochondrial protein.
• Consider using the antibody in ELISA with recombinant DHODH protein or cell lysates; pre-absorption with the immunogen can verify specificity.
• Because DHODH is involved in pyrimidine metabolism, its expression may vary across tissues and cell lines—validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a seamless procurement experience for diagnostic manufacturers and research laboratories, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-DHODH antibody supports studies in nucleotide metabolism and mitochondrial function, complementing our portfolio of metabolic enzyme raw materials. Whether you need rare target sourcing or technical consultation, CamelBio bridges the gap from concept to clinic.