Anti-DHFR Polyclonal Antibody for WB - P00374

Core Product Specifications and Parameters

Biological Background: DHFR Function and Localization

• DHFR catalyzes the NADPH-dependent reduction of 7,8-dihydrofolate to 5,6,7,8-tetrahydrofolate, a critical step in one-carbon metabolism. Related references: PMID:12096917 PMID:15039552 PMID:17569517
• This reaction is essential for de novo thymidylate, glycine, and purine synthesis, providing DNA precursors for nuclear and mitochondrial genomes. Related references: PMID:21876188 PMID:22235121
• DHFR localizes to the cytoplasm, mitochondria, and nucleus, supporting folate metabolism across multiple cellular compartments.
• The enzyme is widely expressed in fetal and adult tissues, with higher levels in the adult brain and whole blood.
• Alternative splicing produces multiple isoforms, and mutations in DHFR are associated with methotrexate resistance and disease variants.
• DHFR binds its own mRNA and that of DHFR2, indicating a potential regulatory role in post-transcriptional gene expression.
• The protein undergoes ubiquitination and has been identified in large-scale proteomic screens.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human DHFR (aa 1-187), suggesting potential reactivity with native protein across species where homology is high (confirmed for human and mouse). Validate in your sample system.
• For Western blotting, expect a band at ~21 kDa. Optimize blocking and detection conditions, and include appropriate loading controls.
• For immunofluorescence/ICC, consider fixation and permeabilization protocols that preserve mitochondrial and nuclear DHFR localization.
• When performing ELISA, recombinantly expressed DHFR or cell lysates can serve as antigens; antibody titration is recommended.
• DHFR expression varies by tissue and cell cycle stage; include relevant positive and negative controls in your experiments.
• As a polyclonal antibody, assess lot-to-lot consistency and consider using a blocking peptide to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a streamlined sourcing pathway for critical IVD raw materials. The DHFR target is essential for studies in folate metabolism, cancer, and antimicrobial research, and we can supply validated polyclonal antibodies, optimized antibody pairs, and custom production services to meet your project needs. Our one-stop platform covers every stage from concept to clinic, ensuring consistent quality and technical support.