Anti-DGUOK Polyclonal Antibody for WB, ELISA - Q16854

Core Product Specifications and Parameters

Biological Background: DGUOK Function and Localization

• DGUOK is a mitochondrial deoxyguanosine kinase (dGK) also known as deoxyadenosine kinase, playing a key role in the mitochondrial salvage pathway.
• It catalyzes the phosphorylation of deoxyguanosine and deoxyadenosine in the mitochondrial matrix, with the highest efficiency for deoxyguanosine (PubMed:11687801, PubMed:17073823, PubMed:23043144). Related references: PMID:11687801 PMID:17073823 PMID:23043144
• In non-replicating cells, where cytosolic dNTP synthesis is down-regulated, mtDNA synthesis depends solely on DGUOK and TK2, and it also phosphorylates certain nucleoside analogs (By similarity).
• DGUOK localizes exclusively to the mitochondrion.
• Expression is ubiquitous, with highest levels in muscle, brain, liver, and lymphoid tissues.
• The protein undergoes alternative splicing and acetylation, and is associated with disease variants leading to mitochondrial DNA depletion syndromes.
• As a kinase, it is a target for antiviral and chemotherapeutic agents.

Experimental Guidance and Technical Tips

• For Western blotting, a band around 32 kDa is expected; validate in appropriate tissue or cell lysates (e.g., high expression in muscle, brain, liver).
• For ELISA, consider using recombinant DGUOK protein as a standard, and note that cross-reactivity with mouse and rat orthologs has been reported.
• Since DGUOK is mitochondrial, ensure proper subcellular fractionation if enrichment is needed.
• Nucleoside analog studies may require phosphorylation activity assays alongside antibody detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with integrated access to high-quality IVD raw materials, technical services, and consulting. This anti-DGUOK antibody supports research into mitochondrial DNA synthesis, nucleoside metabolism, and related diseases. Beyond this polyclonal antibody, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development pipeline.