Anti-DGLUCY Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q7Z3D6

Core Product Specifications and Parameters

Biological Background: DGLUCY Function and Localization

• DGLUCY (also known as C14orf159) is a mitochondrial enzyme that functions as a D-glutamate cyclase, catalyzing the conversion of D-glutamate to 5-oxo-D-proline.
• The protein is localized to the mitochondrial matrix, suggesting a role in amino acid metabolism within this organelle.
• DGLUCY belongs to the lyase class of enzymes, specifically involved in carbon-carbon bond cleavage.
• Alternative splicing of the DGLUCY gene produces multiple transcript variants, contributing to protein diversity.
• It is classified as a reference proteome component and has been identified in proteomics identification studies.
• The protein contains a transit peptide, consistent with its mitochondrial targeting.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 269-616 of human DGLUCY, a region likely to include exposed epitopes; consider this when predicting reactivity with splice variants or orthologs.
• For Western blotting, the predicted molecular weight is 66 kDa; validate band specificity using appropriate positive and negative controls.
• In IF/ICC, confirm mitochondrial localization by co-staining with mitochondrial markers and validating in cell lines expressing DGLUCY.
• For ELISA, the polyclonal nature may enhance sensitivity; optimize coating antigen and antibody dilution for your specific assay format.
• Cross-reactivity with mouse is reported; verify reactivity in your sample system if using mouse tissues or cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like DGLUCY involved in mitochondrial metabolism, we offer sourcing support for rare and specialized reagents to accelerate your assay development. Our one-stop service covers every stage from concept to clinic, ensuring you have the critical raw materials needed for your research and diagnostic applications.