Anti-DGLUCY Monoclonal Antibody for WB, IHC-P, ELISA - Q7Z3D6

Core Product Specifications and Parameters

Biological Background: DGLUCY Function and Localization

• DGLUCY (C14orf159) is a mitochondrial D-glutamate cyclase that specifically converts D-glutamate to 5-oxo-D-proline.
• The protein is localized to the mitochondrial matrix, where it likely participates in amino acid metabolism and redox balance.
• Alternative splicing generates multiple isoforms of DGLUCY, which may have distinct regulatory or functional roles.
• The enzyme belongs to the lyase class, catalyzing the intramolecular cyclization of D-glutamate without requiring cofactors.
• DGLUCY is conserved between human and mouse, with a predicted molecular weight of approximately 66 kDa.
• Proteomics studies have identified DGLUCY in multiple human tissues, supporting its ubiquitous expression and physiological relevance.

Experimental Guidance and Technical Tips

• The immunogen was designed against an internal region (aa 269–616) of human DGLUCY; for applications like Western blot, verify the detected band corresponds to the predicted 66 kDa size in your sample system.
• When using immunohistochemistry (IHC-P), consider testing both frozen and paraffin-embedded sections, as antigen retrieval methods may influence signal quality.
• For ELISA applications, evaluate the antibody’s performance in direct, indirect, or sandwich formats with recombinant DGLUCY or cell lysates.
• Due to the target’s mitochondrial localization, efficient lysis and sample preparation are critical for reproducible detection in whole-cell extracts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research labs with direct access to high-quality IVD raw materials, including validated monoclonal antibodies like this anti-DGLUCY clone, ideal for mitochondrial metabolism and neurological disease research. Beyond single reagents, we constantly provide matched antibody pairs, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your assay development. Leverage our integrated supply chain and technical support to accelerate your projects from concept to clinic.