Anti-DGKZ (Diacylglycerol Kinase Zeta) Rabbit Polyclonal Antibody for WB and ELISA - Q13574

Core Product Specifications and Parameters

Biological Background: Diacylglycerol kinase zeta Function and Localization

• Diacylglycerol kinase zeta (DGK-zeta), encoded by the DGKZ (DAGK6) gene, is a kinase with ANK repeats and zinc-finger domains.
• Converts diacylglycerol (DAG) to phosphatidic acid (PA), regulating the balance of these bioactive lipids and acting as a switch between their signaling pathways. Related references: PMID:15544348, PMID:18004883, PMID:19744926
• Plays a role in the biosynthesis of complex lipids (Probable).
• Exhibits no acyl chain-dependent substrate specificity among diacylglycerol species but can phosphorylate 1-alkyl-2-acylglycerol in vitro with a preference for arachidonoyl-containing species. Related references: PMID:9159104, PMID:15544348, PMID:19744926, PMID:22108654, PMID:22627129
• Negatively regulates T-cell receptor signaling, modulating T cell activation (By similarity).
• Stimulates PIP5KIA activity to promote actin polymerization. Related references: PMID:15157668
• Subcellular localization includes the nucleus, cytoplasm, cytosol, cell membrane, and lamellipodium.
• Tissue specificity: highest expression in brain, with substantial levels in skeletal muscle, heart, and pancreas; predominantly expressed in muscle.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 808–1117) of human DGKZ, which may be suitable for detecting full-length and alternatively spliced isoforms.
• For Western blot (WB), expect a band at approximately 104 kDa; validate in relevant human or mouse sample systems.
• For ELISA, consider testing under both direct and indirect formats, optimizing antibody concentration and blocking conditions.
• Note the reported cross-reactivity with mouse; sequence homology should be examined for other species if needed.
• As a polyclonal antibody, lot-to-lot consistency should be verified for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-DGKZ antibody supports research into lipid signaling pathways, T cell activation, and related diagnostic development. For rare or custom target raw materials, our team offers specialized sourcing and technical consultation.