Anti-DGKA Rabbit Polyclonal Antibody for WB, ELISA - P23743

Core Product Specifications and Parameters

Biological Background: DGKA Function and Localization

• DGKA is an 80 kDa diacylglycerol kinase that converts diacylglycerol (DAG) into phosphatidic acid (PA), modulating the levels of these two critical lipid second messengers (PubMed:15544348, PubMed:2175712). Related references: PMID:15544348, PMID:2175712
• Through this activity, DGKA serves as a molecular switch between DAG- and PA-mediated signaling pathways, which often have opposing effects on cell growth, differentiation, and apoptosis (PubMed:15544348, PubMed:2175712). Related references: PMID:15544348, PMID:2175712
• It also participates in complex lipid biosynthesis and can phosphorylate 1-alkyl-2-acylglycerol (PubMed:15544348) and 1-alkyl-2-acetyl glycerol, contributing to alkyl-lysophosphatidic acid production (PubMed:22627129). Related references: PMID:15544348, PMID:22627129
• DGKA contains zinc finger domains, an ATP-binding site, and EF-hand calcium-binding motifs, suggesting regulation by calcium and zinc.
• The protein resides primarily in the cytoplasm and cytosol.
• It is predominantly expressed in lymphocytes.
• Alternative splicing generates multiple DGKA isoforms.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-200), which includes conserved domains; cross-reactivity with mouse and rat has been observed, but validation in your specific experimental system is recommended.
• For Western blotting, a band around 83 kDa is expected based on the predicted protein weight; using appropriate loading controls and verifying with positive control lysates from lymphocytes may be beneficial.
• For ELISA, optimizing coating antigen concentration and antibody dilution using checkerboard titration can help achieve optimal signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, from validated antibody pairs and optimized polyclonal/monoclonal antibodies to bulk ancillary reagents, supporting your development of immunoassays for lipid kinases like DGKA. Whether you need small-scale evaluation or large-batch production, CamelBio streamlines sourcing from concept to clinic, ensuring consistent quality and supply for your diagnostic or research workflows.