Anti-DGKA Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P23743

Core Product Specifications and Parameters

Biological Background: Diacylglycerol kinase alpha Function and Localization

• Diacylglycerol kinase alpha (DGKA), also known as 80 kDa diacylglycerol kinase or DGK-alpha, is a lipid kinase that phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA).
• By interconverting DAG and PA, DGKA serves as a central switch between signaling pathways involving these two second messengers, which have contrasting cellular effects. Related references: PMID:15544348, PMID:2175712
• DGKA can also phosphorylate 1-alkyl-2-acylglycerol (if it contains an arachidonoyl group) and 1-alkyl-2-acetyl glycerol, contributing to the production of alkyl-lysophosphatidic acid, another bioactive lipid. Related references: PMID:15544348, PMID:22627129
• Plays a role in the biosynthesis of complex lipids (Probable).
• Localizes primarily to the cytoplasm and cytosol.
• Predominantly expressed in lymphocytes, suggesting a role in immune cell signaling.
• Contains conserved domains including zinc-finger motifs, an ATP-binding site, and a calcium-binding region; subject to post-translational acetylation and alternative splicing.
• Theoretical molecular weight is approximately 82.6 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1–200) of human DGKA; consider that epitopes within conserved domains may lead to cross-reactivity with other DGK family members.
• For Western blot, a band at ~83 kDa is expected; validate with appropriate positive controls such as lymphocyte lysates or DGKA-overexpressing cell lines.
• In IHC-P, optimize antigen retrieval and antibody dilution, as epitopes may be masked by fixation.
• ELISA performance may depend on plate coating and detection reagents; pairing with a suitable secondary antibody is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated monoclonal antibodies, recombinant proteins, and ancillary reagents. For diacylglycerol kinase research and related lipid signaling studies, we supply high-quality antibodies like this DGKA Rabbit mAb to support immunoassay development. Reach out for tailored sourcing solutions, bulk orders, or technical consultation.