Anti-DGKA Rabbit Monoclonal Antibody for WB, ELISA - P23743

Core Product Specifications and Parameters

Biological Background: DGKA Function and Localization

• Protein identity: Diacylglycerol kinase alpha (DGKA), also known as 80 kDa diacylglycerol kinase or DGK-alpha, is encoded by the DGKA gene (Gene ID: 1606). Alternative names include DAGK and DAGK1.
• Core function: DGKA catalyzes the conversion of diacylglycerol (DAG) into phosphatidic acid (PA), thereby regulating the balance of these two key second messengers. It acts as a central switch between signaling pathways with opposite biological effects. Related references: PMID:15544348 PMID:2175712
• Biosynthetic role: Additionally, DGKA contributes to the biosynthesis of complex lipids.
• Substrate specificity toward alkyl‑acylglycerols: DGKA can phosphorylate 1‑alkyl‑2‑acylglycerol in vitro, especially when an arachidonoyl group is present. Related references: PMID:15544348
• Production of alkyl‑lysophosphatidic acid: It is also involved in generating alkyl‑lysophosphatidic acid, a bioactive lipid, by phosphorylating 1‑alkyl‑2‑acetyl glycerol. Related references: PMID:22627129
• Subcellular localization: Found in the cytoplasm and cytosol.
• Tissue specificity: Expressed in lymphocytes.
• Structural features: Contains an ATP‑binding domain, zinc‑finger motifs, and repeat regions. The protein undergoes acetylation and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal region (amino acids 1‑200) of human DGKA. This region may contain critical functional domains; validate antibody specificity in your target sample system.
• For WB, expect a band at approximately 83 kDa. Use appropriate loading controls and confirm cross‑reactivity in human, mouse, and rat lysates as indicated.
• ELISA protocols can be used; optimize coating antigen concentration and antibody dilution to achieve the best signal‑to‑noise ratio.
• Because DGKA participates in lipid signaling, consider employing relevant cell stimulation or lipid extraction methods to enhance detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one‑stop IVD raw‑material sourcing partner, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target sourcing. This DGKA antibody supports investigations into diacylglycerol kinase signaling, lipid metabolism, and associated disease models. Partner with us for seamless procurement from concept to clinic.