Anti-DGCR8 Rabbit Monoclonal Antibody for WB, ELISA - Q8WYQ5

Core Product Specifications and Parameters

Biological Background: DGCR8 Function and Localization

• Encoded by the DGCR8 gene (C22orf12, DGCRK6) on chromosome 22, DGCR8 is the RNA-binding subunit of the microprocessor complex essential for primary miRNA processing.
• DGCR8 functions as a molecular anchor that recognizes the dsRNA-ssRNA junction of pri-miRNAs and directs DROSHA to cleave precisely 11 bp away, generating pre-miRNA hairpins.
  Related references:
  PMID:15531877 PMID:15574589 PMID:15589161 PMID:26027739 PMID:26748718
• Heme binding to DGCR8 promotes dimerization and formation of a cooperative trimer that enhances pri-miRNA cleavage activity, while the heme-free monomer exhibits lower activity.
• DGCR8 specifically recognizes N6-methyladenosine (m6A) modifications in pri-miRNAs, a critical determinant for efficient processing.
  Related references:
  PMID:25799998
• In addition to miRNA biogenesis, DGCR8 participates in DNA double-strand break repair by facilitating the recruitment of RNF168 to DNA damage foci.
  Related references:
  PMID:34188037
• DGCR8 is localized primarily to the nucleus and nucleolus, consistent with its roles in RNA processing and genome stability.
• The protein is ubiquitously expressed and subject to post-translational modifications including phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation.

Experimental Guidance and Technical Tips

• When performing Western blot, a band around 86 kDa is expected; validate specificity using siRNA-mediated knockdown or DGCR8-overexpressing lysates.
• For ELISA, the immunogen (amino acids 436-764) can serve as a positive control; consider optimizing coating and detection conditions in the relevant sample matrix.
• Verify cross-reactivity in human and mouse systems as indicated; for other species, test empirically due to sequence conservation.
• As DGCR8 participates in dynamic protein complexes, native or mild denaturing conditions may be required to preserve epitope accessibility in immunoprecipitation-based applications.
• The antibody may also be suitable for immunohistochemistry or immunofluorescence after validation; consider antigen retrieval methods appropriate for nuclear proteins.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies like this DGCR8 Rabbit mAb, and bulk ancillary reagents. Whether you need rare target reagents or support scaling from research to diagnostic kit production, CamelBio bridges the gap from concept to clinic with reliable sourcing and technical consultation.