Anti-DGAT2 Rabbit Polyclonal Antibody for WB, ELISA - Q96PD7

Core Product Specifications and Parameters

Biological Background: DGAT2 Function and Localization

• DGAT2 catalyzes the terminal step in triacylglycerol synthesis, using diacylglycerol and fatty acyl-CoA, and is required for intracellular triglyceride synthesis and storage (PubMed:27184406). Related references: PMID:27184406
• It also functions as an acyl-CoA retinol acyltransferase (ARAT) and can utilize 1-monoalkylglycerol as an acyl acceptor to produce monoalkyl-monoacylglycerol (PubMed:28420705). Related references: PMID:28420705
• In the liver, DGAT2 is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides.
• The protein localizes to the endoplasmic reticulum membrane, lipid droplets, and the perinuclear cytoplasm.
• DGAT2 is predominantly expressed in liver and white adipose tissue, with lower expression in mammary gland, testis, peripheral blood leukocytes, and sebaceous glands.
• As a member of the acyltransferase family, DGAT2 is a multi-pass membrane protein with transferase activity, playing a central role in lipid metabolism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 289-388) of human DGAT2; consider verifying specificity for full-length and potential isoforms.
• For Western blot, a band at approximately 44 kDa is expected; validate detection in relevant samples such as liver or adipose tissue lysates.
• When performing ELISA, pairing with a detection antibody targeting a distinct epitope may improve assay performance.
• Since DGAT2 is an integral membrane protein, use appropriate lysis and sample preparation buffers; avoid boiling to prevent aggregation.
• Cross-reactivity with mouse and rat indicates conserved epitopes, making this antibody suitable for translational studies in rodent models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research labs, and institutes with a comprehensive one-stop sourcing solution for IVD raw materials. Our supply capabilities include optimized monoclonal and polyclonal antibodies, validated antibody pairs, and bulk ancillary reagents tailored for lipid metabolism and metabolic disease research. For specialized targets like DGAT2, CamelBio streamlines procurement to support your entire assay development workflow from concept to clinic.