Anti-DGAT1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O75907

Core Product Specifications and Parameters

Biological Background: DGAT1 Function and Localization

• DGAT1 (Diacylglycerol O-acyltransferase 1), also known as ARAT, DGAT, ARGP1, DIAR7, catalyzes the terminal and only committed step in triacylglycerol synthesis using diacylglycerol and fatty acyl CoA as substrates. Related references: PMID:16214399, PMID:18768481, PMID:28420705
• In the small intestine, DGAT1 is highly expressed in epithelial cells and is essential for the absorption of dietary fats. Related references: PMID:18768481
• In the liver, DGAT1 plays a role in esterifying exogenous fatty acids to glycerol and is required for fat storage. Related references: PMID:16214399
• DGAT1 is present in female mammary glands, where it produces fat in the milk (By similarity).
• The enzyme may be involved in very low density lipoprotein (VLDL) assembly. Related references: PMID:18768481
• As the major acyl-CoA retinol acyltransferase (ARAT) in the skin, DGAT1 maintains retinoid homeostasis and prevents retinoid toxicity. Related references: PMID:16214399
• DGAT1 also exhibits additional acyltransferase activities, including monoacylglycerol acyltransferase (MGAT) and wax synthase activities, and can use 1-monoalkylglycerol as an acyl acceptor. Related references: PMID:28420705
• Subcellular localization: DGAT1 is an endoplasmic reticulum membrane protein.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 201-300 of human DGAT1 (NP_036211.2). This region may be conserved across species, supporting the observed cross-reactivity with mouse and rat.
• For Western blotting, the predicted molecular weight of DGAT1 is ~55 kDa. Validate the antibody with appropriate positive and negative controls in your sample system.
• In immunofluorescence and ELISA applications, consider optimizing the dilution factor and blocking conditions based on the specific sample type and experimental design.
• Cross-reactivity with human, mouse, and rat suggests potential utility in rodent models; however, independent validation in the relevant tissue or cell line is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw-material sourcing, supporting diagnostic manufacturers and researchers from concept to clinic. For lipid metabolism assays targeting DGAT1, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our team can also assist in sourcing rare target raw materials, ensuring reliable supply for your DGAT1 and metabolic research needs.