Anti-DFFA Polyclonal Antibody for WB, ELISA - O00273

Core Product Specifications and Parameters

Biological Background: DFFA Function and Localization

• DFFA, also known as DNA fragmentation factor 45 kDa subunit (DFF-45) or inhibitor of CAD (ICAD), is a key regulator of apoptosis.
• It functions as an inhibitor of the caspase-activated DNase (DFF40/CAD), preventing DNA fragmentation in the absence of apoptotic signals.
• Upon caspase-3 cleavage, DFFA is inactivated, releasing DFF40 to enter the nucleus and execute DNA fragmentation, a hallmark of apoptosis.
• DFFA is primarily localized in the cytoplasm, where it complexes with DFF40 to maintain its inactive state.
• The protein undergoes alternative splicing, producing multiple isoforms with potentially distinct regulatory roles.
• Post-translational modifications, including phosphorylation and acetylation, may modulate DFFA activity and stability.
• DFFA is implicated in both caspase-dependent and independent cell death pathways, making it a critical target for apoptosis research.

Experimental Guidance and Technical Tips

• The immunogen (full-length human DFFA) spans amino acids 1-331, covering the majority of the protein, which may provide robust detection of the 37 kDa band in western blotting.
• For western blot, consider using a positive control such as apoptotic cell lysates where caspase cleavage alters DFFA migration patterns.
• This polyclonal antibody may recognize multiple DFFA isoforms due to alternative splicing; validate specificity using isoform-specific controls if needed.
• For ELISA, the antibody can be used for direct or indirect detection schemes; optimize coating and blocking conditions for your specific assay.
• Cross-reactivity with mouse DFFA has been reported; verify consistency in your experimental systems.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated B2B IVD raw-material partner, CamelBio streamlines your sourcing of high-quality antibodies and ancillary reagents. For apoptosis research, we offer validated anti-DFFA antibodies along with complementary antibodies, recombinant proteins, and assay development support to accelerate your diagnostic pipeline. Contact us to explore custom bulk orders and specialized reagent sourcing for your unique assay requirements.