Anti-DEXI Polyclonal Antibody for WB, ELISA - O95424

Core Product Specifications and Parameters

Biological Background: Dexamethasone-Induced Protein (DEXI) Function and Localization

• DEXI, also known as MYLE, is a dexamethasone-induced protein encoded by the DEXI gene in humans (GeneID 28955).
• Tissue specificity: DEXI is expressed at highest levels in the heart, with additional expression in brain, liver, pancreas, placenta, and lung.
• Upregulated in emphysematous lung compared to normal lung, suggesting a potential role in pulmonary disease pathology.
• Classified as a reference proteome component (UniProtKB entry O95424), indicative of the high quality of its protein-level annotation.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight of DEXI is 10 kDa; therefore, a high-percentage polyacrylamide gel (e.g., 12-15%) and thorough transfer optimization may be considered to retain the small protein effectively.
• Consider validating the antibody in human heart or lung tissue lysates, where endogenous DEXI expression is reported to be relatively abundant.
• For ELISA, the immunogen (recombinant fusion protein covering amino acids 1-95) can serve as a positive control; confirm assay conditions using recombinant DEXI protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, from validated antibody pairs to bulk ancillary reagents, supporting diagnostic manufacturers and researchers. Our DEXI polyclonal antibody provides a reliable tool for studies in cardiac and pulmonary disease research, and we can assist with sourcing rare targets and custom reagent development.