Anti-DET1 Polyclonal Antibody for WB, ELISA - Q7L5Y6

Core Product Specifications and Parameters

Biological Background: DET1 Function and Localization

• DET1 (De-etiolated-1 homolog) is a nuclear protein that functions as a component of the DCX DET1-COP1 E3 ubiquitin ligase complex.
• The DCX DET1-COP1 complex is essential for ubiquitination and subsequent proteasomal degradation of target proteins, including JUN.
• DET1 exhibits high expression in ovary, certain lymphoid organs, and resting leukocytes, suggesting tissue-specific regulatory roles.
• The protein is subject to alternative splicing and has a resolved 3D structure, indicating conserved structural features.
• Keywords associate DET1 with the ubiquitin conjugation pathway, nuclear localization, and reference proteome.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fusion protein spanning amino acids 15-240 of human DET1, targeting the N-terminal region.
• For Western blot, a band at approximately 64 kDa is expected in human samples; include appropriate positive controls such as ovary or lymphoid tissue lysates where DET1 is highly expressed.
• Cross-reactivity with mouse and rat suggests epitope conservation; consider performing species-specific validation if these species are used.
• In ELISA, consider using the immunogen peptide or full-length DET1 protein as a coating antigen to assess binding specificity.
• This antibody is categorized as a polyclonal antibody, which may offer robust detection but could exhibit lot-to-lot variability; validate each new batch in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials, including validated antibody pairs and polyclonal antibodies like this anti-DET1 reagent. For projects focusing on ubiquitination pathways or cancer research, we can also source rare-target antibodies and bulk ancillary reagents to support your entire development pipeline.