Anti-Desmoplakin Polyclonal Antibody for WB, IHC-P, ELISA - P15924

Core Product Specifications and Parameters

Biological Background: Desmoplakin Function and Localization

• Desmoplakin (DP) is a critical component of desmosome cell-cell junctions, positively regulating cellular adhesion.
  Related references:
  PMID:25733715
• Acts as a keratin intermediate filament anchor, organizing the cytoplasmic keratin network to maintain cell integrity.
• Promotes microtubule-mediated trafficking of GJA1/CX43 and gap junction intercellular communication.
  Related references:
  PMID:25225338
• Essential for dermal epithelial barrier adhesion and structural organization.
  Related references:
  PMID:26604139
• Regulates keratinocyte differentiation via COP9 signalosome complex interaction and EGFR deneddylation.
  Related references:
  PMID:28891468
• Maintains protein stability of desmosomal components (DSG1, DSG2, DSC2, etc.).
  Related references:
  PMID:17475244 | PMID:26073755
• Found at cell junctions (desmosomes), cell membrane, cytoplasm, nucleus, and axon growth cones.
• Expressed in heart, skin keratinocytes, oral mucosa, and arrector pili muscle; considered an obligate desmosome constituent.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal region (aa 1–100). Consider this when interpreting signals in samples where Desmoplakin may undergo proteolytic cleavage or isoform variation.
• For WB, note the large protein size (~332 kDa). Use low-percentage gels (e.g., 6% or gradient) and extended transfer times to ensure efficient membrane binding.
• IHC-P applications: Known positive tissues include skin (keratinocytes) and cardiac muscle. Optimize antigen retrieval and antibody dilution empirically.
• ELISA: The peptide-based immunogen may work well for direct or capture ELISA; validate against recombinant protein or tissue lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined procurement platform for IVD raw materials, specializing in antibodies like this anti-Desmoplakin polyclonal antibody. We supply validated antibody pairs, bulk ancillary reagents, and can source rare-target research tools, supporting your desmosome and cell adhesion investigations from discovery to diagnostic development.