Anti-Desmoglein 3 Rabbit Polyclonal Antibody for WB and ELISA - P32926

Core Product Specifications and Parameters

Biological Background: Desmoglein 3 Function and Localization

• Desmoglein-3 is a calcium‑dependent cadherin family member (CDHF6) that functions as a core component of desmosomes, mediating cell‑cell adhesion in epithelial tissues.
• It is essential for anchoring telogen hair clubs in the hair follicle and for maintaining the epithelial barrier by promoting desmosome‑mediated intercellular attachment between suprabasal and basal cells. Related references: PMID:9701552
• DSG3 participates in mechanotransduction by relaying mechanical strain signals; it is required for YAP1 localization at the plasma membrane and for adherens and desmosome junction assembly in keratinocytes under force. Related references: PMID:31835537
• The cytoplasmic pool of DSG3 modulates cell migration by inhibiting p38MAPK signaling and influences tight junction organization via cortical actin bundling and RAC1. Related references: PMID:26763450 PMID:22796473
• It is predominantly expressed in the basal and spinous layers of the epidermis, esophageal mucosa, tongue, and tonsil, with lower levels in granular layer and between lateral margins of hair follicle outer root sheath cells. Related references: PMID:19717567 PMID:22294297 PMID:30528827 PMID:24220297 PMID:9701552 PMID:16740002
• Key post‑translational features include calcium‑binding, glycosylation, and cleavage on pairs of basic residues (UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 749–999, a region encompassing the intracellular domain; the antibody may be suitable for detecting total DSG3 via WB and ELISA. Verify specificity in your sample system.
• For WB, consider using whole‑cell lysates from human keratinocyte lines (e.g., HaCaT) or esophageal mucosa, as DSG3 is highly expressed there.
• Sample preparation: include protease inhibitors to prevent degradation. Run SDS‑PAGE under reducing conditions; predicted band ~108 kDa.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one‑stop sourcing partner for IVD raw materials, offering a comprehensive portfolio of validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For desmosome biology and pemphigus vulgaris research, we provide high‑quality anti‑Desmoglein 3 reagents and can source rare‑target raw materials to support your diagnostic development pipeline.