Anti-Desmoglein 3 Rabbit Monoclonal Antibody for WB and ELISA - P32926

Core Product Specifications and Parameters

Biological Background: Desmoglein-3 Function and Localization

• Desmoglein-3 (DSG3), also known as 130 kDa pemphigus vulgaris antigen (PVA) and cadherin family member 6, is a calcium-dependent transmembrane glycoprotein that serves as a core component of desmosomal junctions, critically mediating cell-cell adhesion in keratinocytes.
• It is required for positive regulation of cellular adhesion and is essential for desmosome-mediated intercellular attachment in stratified epithelia (PubMed:31835537). Related references: PMID:31835537
• Essential for the anchoring of telogen hairs in the hair follicle via desmosome-mediated adhesion of cells surrounding the hair club and the basal layer of the outer root sheath epithelium (PubMed:9701552). Related references: PMID:9701552
• May contribute to the protein stability of desmosomal plaque components, including desmoplakin (DSP), junction plakoglobin (JUP), and plakophilins (PKP1, PKP2, PKP3) (PubMed:22294297). Related references: PMID:22294297
• Under mechanical strain, Desmoglein-3 facilitates YAP1 localization at the plasma membrane through a complex with PKP1 and 14-3-3γ (YWHAG), potentially promoting YAP1-dependent transcription and cell proliferation (PubMed:31835537). Related references: PMID:31835537
• The cytoplasmic pool of Desmoglein-3 is required for proper localization of CDH1 (E-cadherin) and CTNNB1 (β-catenin) at developing adherens junctions, likely via modulation of SRC kinase activity (PubMed:22294297). Related references: PMID:22294297
• Inhibits keratinocyte migration by suppressing p38 MAPK signaling, thereby moderating wound healing responses (PubMed:26763450). Related references: PMID:26763450
• Localizes to cell membrane, desmosomes, tight junctions, and cytoplasm, and is predominantly expressed in the basal and spinous layers of the epidermis, mucosa, esophageal epithelium, and the outer root sheath of hair follicles.

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against a synthetic peptide corresponding to amino acids 900–999 of human Desmoglein-3 (P32926), which lies within the cytoplasmic domain. Consider that the epitope is intracellular and may require permeabilization for immunofluorescence or immunohistochemistry applications.
• Western blot: For WB, expect a band at ~108 kDa corresponding to full-length Desmoglein-3. Optimize gel percentage and transfer conditions; a 6-8% SDS-PAGE gel is typically suitable for high-molecular-weight proteins.
• ELISA: This antibody is suitable for ELISA-based detection. Validate the assay conditions using appropriate positive and negative controls, including recombinant Desmoglein-3 or cell lysates from expressing cells.
• Species reactivity: Confirmed reactive with human. Cross-reactivity with other species is not reported; if using non-human samples, validate reactivity experimentally.
• General notes: As a rabbit monoclonal antibody, it offers consistent lot-to-lot performance. Store according to the manufacturer's recommendation and avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, offering a comprehensive portfolio from validated monoclonal antibodies to bulk reagents. This anti-Desmoglein-3 antibody supports research in cell adhesion, dermatology, and autoimmune disorders like pemphigus. Leverage our expertise to streamline your diagnostic development pipeline with high-quality, reliable materials.