Anti-Desmoglein 2 Rabbit Monoclonal Antibody for WB, ELISA - Q14126

Core Product Specifications and Parameters

Biological Background: Desmoglein 2 Function and Localization

• Desmoglein-2 is a core component of desmosomal cell-cell junctions, essential for strong intercellular adhesion in epithelial and cardiac tissues. Related references: PMID:17559062, PMID:38395410

• It maintains pluripotency by regulating epithelial-to-mesenchymal transition (EMT) and mesenchymal-to-epithelial transition (MET) through sequestration of CTNNB1 (β-catenin) at cell contacts, preventing its nuclear signaling. Related references: PMID:29910125

• Desmoglein-2 promotes the pluripotency and multi-lineage differentiation potential of hematopoietic stem cells and regulates endothelial cell sprouting by mediating junctional association of cortical actin with CDH5. Related references: PMID:27338829

• Independent of its adhesive role, desmoglein-2 positively modulates CSK and EGFR activation by sequestering them outside lipid rafts and disrupts CAV1 localization, influencing cell signaling. Related references: PMID:26918609

• In cardiomyocytes, it localizes to intercalated discs and maintains desmosome integrity, supporting cardiac conduction and chamber function.

• In the kidney, desmoglein-2 limits inflammatory infiltration and apoptotic injury in tubular epithelial cells by preserving the epithelial barrier. Related references: PMID:38395410

• Subcellularly, it is found at the cell membrane within desmosomes, in the cytoplasm, and a secreted form exists, reflecting its dynamic roles.

• Tissue expression is broad, including undifferentiated stem cells, hematopoietic progenitors, endothelial cells of arterioles/venules, cardiomyocytes, pancreatic islet cells, and kidney tubular epithelia; expression often decreases upon differentiation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C‑terminal intracellular domain (aa 1001‑1118), suggesting the antibody recognizes the full‑length desmoglein‑2 protein; confirm reactivity in your model system.
• For Western blotting, the predicted molecular weight is ~122 kDa; use appropriate gel percentage and transfer conditions for large transmembrane proteins.
• As a rabbit monoclonal antibody, it provides consistent batch‑to‑batch reproducibility; optimize dilution for your specific WB or ELISA protocol.
• When using human‑specific reactivity, validate cross‑reactivity in other species if required, and consider sequence alignment of the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, including specialized targets like desmoglein‑2 for cell adhesion and cardiac research. Whether you need rare‑target sourcing or full‑process technical support, CamelBio streamlines your procurement and accelerates assay development.