Anti-Desmocollin 3 Rabbit Monoclonal Antibody for WB, ELISA - Q14574

Core Product Specifications and Parameters

Biological Background: Desmocollin 3 Function and Localization

• Encodes Desmocollin 3, a calcium-dependent cadherin family member (alternative names: DSC3, CDHF3, DSC4, HT-CP) that is a core component of desmosome cell-cell junctions.
• Functions in positive regulation of cellular adhesion and is required for cell-cell adhesion in the epidermis, thereby maintaining dermal cohesion and barrier function. Related references: PMID:19717567
• Plays a critical role in anchoring the telogen hair shaft by mediating adhesion of epithelial cell layers surrounding the hair club (By similarity).
• Expressed throughout the basal and spinous layers of the epidermis with weak expression in the granular layer, and also detected in buccal mucosa, esophagus, and cervix. Related references: PMID:19717567 PMID:7665906
• Localizes to the cell membrane at desmosomal junctions and is also found in the cytoplasm.
• Undergoes alternative splicing and proteolytic cleavage, and is subject to N-linked glycosylation.
• Contains cadherin repeats and calcium-binding motifs, classifying it as a metal-binding transmembrane glycoprotein.
• Essential for embryo compaction and early development in mammals (By similarity).

Experimental Guidance and Technical Tips

• For Western blot, consider using whole-cell lysates from human epidermal tissues or cell lines (e.g., HaCaT) under reducing conditions; the expected molecular weight is approximately 100 kDa. Additional bands may arise from post-translational modifications or alternative splicing.
• In ELISA, the antibody may perform well given its peptide immunogen; however, optimization of coating conditions and detection systems is recommended.
• Cross-reactivity is confirmed for human; for other species, homology should be verified experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibodies, antigens, and ancillary reagents for diagnostic workflows. This anti-Desmocollin 3 monoclonal antibody supports research into desmosomal adhesion and skin barrier disorders. For custom bulk orders or matched detection pairs, contact our technical team.