Anti-Desmocollin 1 Monoclonal Antibody for WB, ELISA - Q08554

Core Product Specifications and Parameters

Biological Background: Desmocollin-1 Function and Localization

• Desmocollin-1, also known as cadherin family member 1 or desmosomal glycoprotein 2/3 (DG2/DG3), is encoded by the DSC1 gene (CDHF1) and belongs to the cadherin superfamily of cell adhesion molecules.
• As a key component of desmosomes, it facilitates cell-cell adhesion by interacting with desmogleins in the intercellular space, providing mechanical strength to tissues subjected to stress.
• It is required for maintaining adhesion strength between the granular layers of the epidermis, playing a critical role in epidermal integrity and barrier function.
• Desmocollin-1 moderates epidermal proliferation and differentiation, contributing to the proper formation and maintenance of the skin and hair follicles into adulthood.
• The protein localizes to the cell membrane, specifically at desmosomal junctions.
• Tissue expression is predominantly in the epidermis, with lower levels detected in lymph node and tongue.
• Post-translational modifications include cleavage on pairs of basic residues, glycosylation, and phosphorylation, which may regulate its function and stability.
• Alternative splicing of the DSC1 gene produces multiple isoforms, potentially regulating desmosome assembly and function.

Experimental Guidance and Technical Tips

• For western blotting, consider using mild detergent lysis buffers to preserve desmosomal complexes; the predicted molecular weight is ~100 kDa, but post-translational modifications may alter migration.
• Validate antibody specificity in relevant human epidermal lysates or rat skin tissue, as cross-reactivity has been observed.
• For ELISA, a recombinant Desmocollin-1 protein fragment (e.g., amino acids 824-894) may serve as a suitable positive control.
• When probing for desmosomal proteins, note that mechanical disruption during sample preparation can affect detection; gentle homogenization is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and labs with comprehensive access to IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. This anti-Desmocollin 1 antibody supports research in dermatology, cell adhesion, and desmosome-related disorders. As your sourcing partner, CamelBio streamlines procurement from concept to clinic with reliable supply and technical support.