Anti-Desmin Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P17661

Core Product Specifications and Parameters

Biological Background: Desmin Function and Localization

• Desmin is a muscle-specific type III intermediate filament protein essential for sarcomere organization. It interconnects Z-disks, linking myofibrils to the sarcolemmal cytoskeleton, nucleus, and mitochondria, providing mechanical strength during muscle contraction (PubMed:25358400) Related references: PMID:25358400
• In adult striated muscle, desmin forms a fibrous network at the Z-line periphery that connects myofibrils to each other and to the plasma membrane (PubMed:24200904, PubMed:25394388, PubMed:26724190) Related references: PMID:24200904, PMID:25394388, PMID:26724190
• Desmin may function as a sarcomeric microtubule-anchoring protein by associating with detyrosinated tubulin-alpha chains, inducing microtubule buckling and enhancing mechanical resistance to contraction
• It contributes to nuclear membrane integrity through anchoring at the cell tip and nuclear envelope, maintaining microtubule-derived intracellular forces
• Subcellularly, desmin localizes to the cytoplasm, sarcomere Z-lines, cell membrane (sarcolemma), nucleus, cell tip, and nuclear envelope
• Mutations in DES are associated with desmin-related myopathy, cardiomyopathy, and limb-girdle muscular dystrophy, highlighting its critical role in muscle disease

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-90) of human Desmin; consider epitope accessibility in your assay design
• This polyclonal antibody may detect multiple isoforms or degradation products; validate specificity in your target tissue or cell line
• Recommended applications include WB, IF-P, IHC-P, and ELISA; optimize antibody dilution and sample preparation for each technique
• Cross-reactivity with human, mouse, and rat samples enables multispecies studies; confirm performance in the relevant organism
• For paraffin-embedded IHC, antigen retrieval may enhance signal; test appropriate retrieval methods for your sample type

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research teams with integrated access to premium IVD raw materials, including validated antibodies like this anti-Desmin pAb. We streamline your procurement process—from optimized monoclonal/polyclonal antibodies and validated pairs to bulk ancillary reagents and hard-to-find target sourcing—accelerating your assay development for intermediate filament and sarcomere research.