Anti-Dermcidin (DCD) Rabbit Polyclonal Antibody for WB, ELISA - P81605

Core Product Specifications and Parameters

Biological Background: Dermcidin Function and Localization

• Dermcidin (DCD), also known as preproteolysin, is a secreted antimicrobial protein encoded by the DCD gene (AIDD, DSEP). It undergoes alternative splicing to generate multiple isoforms.
• It is found in sweat and exhibits potent antimicrobial activity against a range of pathogens, including E. coli, E. faecalis, S. aureus, and C. albicans, with optimal activity at sweat-like pH and salt conditions (PubMed:11694882, PubMed:23426625). Related references: PMID:11694882 PMID:23426625
• Dermcidin assembles into homohexameric complexes that insert into pathogen membranes, forming anion channels that disrupt the transmembrane potential and lead to microbial death (PubMed:23426625). Related references: PMID:23426625
• Beyond antimicrobial function, DCD exhibits proteolytic activity, cleaving peptide bonds on the C-terminal side of arginine and, to a lesser extent, lysine residues (PubMed:17448443). Related references: PMID:17448443
• It also promotes neuron survival and possesses phosphatase activity, and may bind IgG, suggesting roles in neural protection and immune modulation (PubMed:9736629). Related references: PMID:9736629
• Subcellularly, DCD is secreted into sweat and other biological fluids; it can also associate with membranes. It has been detected in urine at the protein level.
• Constitutively expressed in eccrine sweat gland cells, with secretion into sweat at concentrations of 1–10 µg/ml.
• Key post-translational modifications include acetylation and glycosylation (proteoglycan), and it binds metal ions such as zinc and manganese, which are relevant for its structure and function.

Experimental Guidance and Technical Tips

• The immunogen was a recombinant protein covering amino acids 20-110 of human DCD (NP_444513.1). For WB, consider testing a range of primary antibody dilutions (e.g., 1:500–1:2000) and validate in your specific sample matrix.
• As DCD is a secreted protein, optimal detection in WB may require analysis of conditioned media or urea-containing extracts. For tissue samples, ensure thorough homogenization to release the soluble 11kDa form.
• For ELISA, direct or sandwich formats may be evaluated; pairing with a matched detection antibody could improve assay sensitivity. Validate in relevant biological fluids (e.g., sweat, urine) given cross-reactivity with human and mouse orthologs.
• Cross-reactivity with mouse is reported, but always confirm species-specific detection in your experimental system using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw-material sourcing with a comprehensive portfolio of validated antibodies, antigens, and ancillary reagents. This anti-Dermcidin antibody supports research into antimicrobial peptides, sweat gland biology, and cancer biomarker development. Backed by CamelBio's integrated technical and supply chain expertise, we help diagnostic manufacturers and labs accelerate from concept to clinic.